Description

8-Azido-2'-Deoxyadenosine-5'-Triphosphate (CAS NO 117679-32-0) is a chemically modified nucleotide triphosphate analog featuring a reactive azido group at the 8-position of the adenine base. This modification is critical for its primary role as a versatile building block in bioorthogonal chemistry, enabling click chemistry reactions for labeling and crosslinking applications. It is an essential reagent for researchers in molecular biology, biochemistry, and pharmaceutical development who require precise, covalent tagging of nucleic acids.

Application

• Click Chemistry Labeling: Used as a substrate for copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) to label DNA or RNA with fluorophores, biotin, or other probes.
• Nucleic Acid Crosslinking Studies: Enables the study of protein-DNA/RNA interactions and nucleic acid structure through photoaffinity or chemical crosslinking.
• Next-Generation Sequencing (NGS): Serves as a modified nucleotide for specialized sequencing applications, including the development of novel sequencing-by-synthesis methodologies.
• Probe Development: Incorporated into oligonucleotides to create highly specific detection probes for diagnostic assays and in situ hybridization.
• Enzyme Mechanism Studies: Acts as a substrate analog to investigate the kinetics and mechanisms of DNA polymerases, kinases, and other nucleic acid-metabolizing enzymes.
• Materials Science: Used in the synthesis of functionalized biomaterials and DNA nanostructures where covalent attachment points are required.

Basic Information

Product Name	8-Azido-2'-Deoxyadenosine-5'-Triphosphate
CAS No.	117679-32-0
Molecular Formula	C10H14N8O12P3
Molecular Weight	555.16 g/mol
Synonyms	8-N3-dATP; 8-Azido-2'-deoxyadenosine 5'-triphosphate; 8-Azido-dATP; Azido-dATP; 2'-Deoxy-8-azidoadenosine-5'-triphosphate; 8-Azidoadenosine 5'-(tetrahydrogen triphosphate), 2'-deoxy-; 8-Azido-2'-deoxyadenosine triphosphate
EINECS	Contact for details

Quality Control

Our 8-Azido-2'-Deoxyadenosine-5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, essential for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to rigorous in-process controls and final release testing to meet the exacting standards required for life science research and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity	Suitable for enzyme incorporation assays
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.