Description

1-Arabinofuranosyl-5-(4-Azidostyryl)Uracil 5'-Triphosphate is a specialized nucleotide analog triphosphate derivative, engineered for advanced biochemical research and diagnostic applications. Its core value lies in its unique structure, which incorporates a photoreactive azido group and a modified arabinofuranosyl sugar, making it a critical reagent for photoaffinity labeling, cross-linking studies, and the development of novel molecular probes. This compound is essential for researchers and developers in the fields of nucleic acid chemistry, enzymology, and next-generation sequencing technology, where precise molecular tagging and detection are paramount. Its design supports the creation of highly specific tools for investigating biomolecular interactions and mechanisms.

Application

• Photoaffinity Labeling Probes: Acts as a key substrate for cross-linking and identifying nucleic acid-protein interactions and enzyme active sites.
• Next-Generation Sequencing (NGS): Used in the development of novel sequencing-by-synthesis chemistries and specialized nucleotide terminators.
• Enzymatic Synthesis: Serves as a modified nucleotide triphosphate for polymerases in the synthesis of labeled or functionalized DNA/RNA strands.
• Molecular Diagnostics: Incorporated into probes for highly sensitive detection systems, including real-time PCR and microarray platforms.
• Bioconjugation & Click Chemistry: The azido group enables efficient, bio-orthogonal conjugation via copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC).
• Antiviral & Anticancer Research: Investigated as a potential modified nucleoside analog for probing metabolic pathways and therapeutic mechanisms.

Basic Information

Product Name	1-Arabinofuranosyl-5-(4-Azidostyryl)Uracil 5'-Triphosphate
CAS No.	117591-28-3
Molecular Formula	C22H24N6O15P3
Molecular Weight	720.39 g/mol
Synonyms	5-(4-Azidostyryl)-araUTP; Ara-5-(4-Azidostyryl)Uracil Triphosphate; 5-(4-Azidostyryl)-1-β-D-arabinofuranosyluracil 5'-triphosphate; 5-(p-Azidostyryl)araUTP; Azidostyryl araUTP; Modified Nucleotide Triphosphate; Photoactivatable Nucleotide; N3-AraUTP analog (azido derivative)
EINECS	Contact for details

Quality Control

Our production of 1-Arabinofuranosyl-5-(4-Azidostyryl)Uracil 5'-Triphosphate adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. We provide detailed Certificates of Analysis (COA) with every shipment, documenting critical parameters such as purity, absence of nucleases, and performance in enzymatic assays. Our quality system is designed to meet the exacting standards required for life science research, diagnostic development, and pharmaceutical R&D.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, it is recommended to store the product desiccated under an inert atmosphere. Aliquot into single-use vials to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (as triphosphate)
Water Content (KF)	≤ 3.0%
Enzymatic Incorporation Test	Suitable for use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.