Description

N(4)-(n-(6-Trifluoroacetylamidocaproyl)-2-Aminoethyl)-5'-o-Dimethoxytrityl-5-Methyl-2'-Deoxycytidine-3'-N,n-Diisopropylmethylphosphoramidite is a sophisticated, high-purity phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise incorporation of a modified 5-methylcytosine base, a critical feature for enhancing the stability and biological activity of synthetic DNA strands. It is primarily needed by pharmaceutical R&D teams, diagnostic developers, and biotechnology companies engaged in creating advanced therapeutics, including antisense oligonucleotides, aptamers, and primers for sensitive PCR applications.

Application

• Oligonucleotide Synthesis: As a key building block (phosphoramidite) in automated DNA/RNA synthesizers for the production of modified oligonucleotides.
• Therapeutic Development: For the research and manufacturing of antisense drugs, siRNA, and other nucleic acid therapeutics where 5-methylcytosine modification is required.
• Diagnostic Probes: In the creation of high-specificity probes and primers for PCR, qPCR, and next-generation sequencing applications.
• Basic Research: Used in academic and industrial laboratories to study DNA methylation, gene expression, and epigenetic mechanisms.
• Aptamer Production: For synthesizing chemically modified DNA aptamers with improved binding affinity and nuclease resistance for diagnostic and therapeutic use.

Basic Information

Product Name	N(4)-(n-(6-Trifluoroacetylamidocaproyl)-2-Aminoethyl)-5'-o-Dimethoxytrityl-5-Methyl-2'-Deoxycytidine-3'-N,n-Diisopropylmethylphosphoramidite
CAS No.	117032-52-7
Molecular Formula	C53H67F3N6O9P
Molecular Weight	1020.11 g/mol
Synonyms	5'-O-DMT-5-Methyl-2'-deoxycytidine CE phosphoramidite; 5-Methyl-dC CE Phosphoramidite; N4-(6-Trifluoroacetylamidocaproyl)-5-methyl-2'-deoxycytidine-3'-phosphoramidite; 5-Me-dC-CE Phosphoramidite; 5-Methylcytosine CE Phosphoramidite; dC(Me) CE Phosphoramidite; 2'-Deoxy-5-methylcytidine CE phosphoramidite derivative
EINECS	Contact for details

Quality Control

Our N(4)-(n-(6-Trifluoroacetylamidocaproyl)-2-Aminoethyl)-5'-o-Dimethoxytrityl-5-Methyl-2'-Deoxycytidine-3'-N,n-Diisopropylmethylphosphoramidite is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is rigorously tested via HPLC, NMR, and mass spectrometry to confirm identity, purity, and reactivity. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your GMP-compliant or research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will compromise its stability and coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Coupling Efficiency Test	≥ 98.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.