Description

Thymidine 3'-o-(Methanephosphonothioate) is a chemically modified nucleoside, specifically a phosphorothioate derivative of thymidine. This compound is a critical building block and reagent in the synthesis of oligonucleotides with sulfur-modified backbones, which are essential for advanced research in molecular biology and therapeutic development. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and academic research institutions focused on antisense oligonucleotide (ASO) development, siRNA synthesis, and nucleic acid chemistry.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A key phosphoramidite precursor for creating oligonucleotides with nuclease-resistant phosphorothioate linkages, used in therapeutic and diagnostic applications.
• siRNA and RNAi Research: Used in the solid-phase synthesis of modified siRNA strands to enhance stability and cellular uptake.
• Molecular Biology Probes: Manufacture of labeled and unlabeled DNA probes for hybridization, sequencing, and diagnostic assays.
• Pharmaceutical Intermediate: Serves as a vital intermediate in the development of novel nucleotide-based drugs targeting genetic diseases, cancers, and viral infections.
• Bioconjugation and Labeling: Facilitates the site-specific introduction of thioate groups or other functionalities for conjugating oligonucleotides to peptides, dyes, or delivery vehicles.
• Structural and Mechanistic Studies: Employed in biochemical research to study enzyme mechanisms (e.g., polymerases, nucleases) and nucleic acid-protein interactions.

Basic Information

Product Name	Thymidine 3'-o-(Methanephosphonothioate)
CAS No.	117020-20-9
Molecular Formula	C11H17N2O7PS
Molecular Weight	352.30 g/mol
Synonyms	3'-O-(Methanephosphonothioyl)thymidine; Thymidine 3'-(O-methyl phosphorothioate); 3'-Thymidyl methylphosphonothioate; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione 3'-(O-methyl phosphorothioate); 2'-Deoxythymidine 3'-(methylphosphonothioate); dT 3'-(methylphosphonothioate); 3'-O-(Methylphosphonothioyl)-2'-deoxythymidine
EINECS	Contact for details

Quality Control

Our Thymidine 3'-o-(Methanephosphonothioate) is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and pharmaceutical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines to support our customers' regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.