Description

Adenosine 3',5'-Cyclic Monophosphate, P1-(2-Nitrophenyl)Ethyl Ester is a chemically modified, cell-permeable derivative of cyclic AMP (cAMP), a fundamental secondary messenger in cellular signaling. This compound is specifically engineered for research applications where precise control and delivery of cAMP signaling is required. It is a critical tool for researchers in pharmaceutical development, biochemistry, and molecular biology studying signal transduction pathways, enzyme regulation, and cellular response mechanisms.

Application

• Biochemical Research: As a photolabile, "caged" cAMP analog for precise temporal and spatial control of intracellular cAMP levels in live-cell imaging and kinetic studies.
• Signal Transduction Studies: Investigating the role of Protein Kinase A (PKA) and other cAMP-dependent pathways in cellular processes like metabolism, gene expression, and ion channel function.
• Neuroscience & Cardiology Research: Exploring cAMP-mediated effects on neuronal plasticity, cardiac muscle contraction, and heart rate regulation.
• Drug Discovery & Screening: Used in high-throughput assays to identify and characterize novel compounds that modulate adenylate cyclase or phosphodiesterase activity.
• Enzymology: Serving as a substrate or modulator in studies of phosphodiesterases and kinases to understand enzyme kinetics and inhibition.
• Cell Biology: Probing the mechanisms of hormone action, cellular differentiation, and apoptosis triggered by cAMP elevation.

Basic Information

Item	Detail
Product Name	Adenosine 3',5'-Cyclic Monophosphate, P1-(2-Nitrophenyl)Ethyl Ester
CAS No.	116271-32-0
Molecular Formula	C18H20N6O9P
Molecular Weight	504.36 g/mol
Synonyms	cAMP, P1-(2-Nitrophenyl)ethyl ester; 2-Nitrophenylethyl-cAMP; NPE-cAMP; (2-Nitrophenyl)ethyl adenosine-3',5'-cyclic monophosphate; 8-(2-Nitrophenyl)adenosine-3',5'-cyclic monophosphate; Photolabile cAMP derivative; Caged cAMP (2-NPE ester); Adenosine cyclic 3',5'-(hydrogen phosphate) 2-nitrophenylethyl ester
EINECS	Contact for details

Quality Control

Our Adenosine 3',5'-Cyclic Monophosphate, P1-(2-Nitrophenyl)Ethyl Ester is produced under stringent conditions suitable for research use. Each batch undergoes comprehensive analytical testing including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometric analysis. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research specifications. For specific purity grades or custom analytical requirements, please contact our technical team.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and storage under an inert atmosphere. Allow the sealed container to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 3.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.