Description

1-(2-Nitro)Phenylethyl-P(3)-Guanylyl Imidodiphosphate is a specialized nucleotide analog, a high-purity biochemical reagent designed for advanced research and development. This compound is critical for studying enzyme kinetics, signal transduction pathways, and nucleotide-dependent processes due to its role as a non-hydrolyzable GTP analog. It is primarily utilized by pharmaceutical researchers, academic institutions, and biotechnology companies engaged in molecular biology, enzymology, and drug discovery programs.

Application

• Enzymology Research: Used as a non-hydrolyzable GTP analog to study the mechanisms of GTP-binding proteins (G-proteins), GTPases, and other nucleotide-dependent enzymes.
• Signal Transduction Studies: Essential for investigating cellular signaling pathways, including those involving Ras, Rab, and other small GTPase superfamily members.
• Drug Discovery & Screening: Serves as a key reagent in high-throughput screening assays to identify inhibitors or modulators of GTPase activity for therapeutic development.
• Biochemical Assay Development: Employed in the design and validation of sensitive in vitro assays to measure enzyme activity and protein interactions.
• Academic & Pharmaceutical R&D: A fundamental tool for basic research in molecular biology and for target validation in pre-clinical drug development pipelines.

Basic Information

Product Name	1-(2-Nitro)Phenylethyl-P(3)-Guanylyl Imidodiphosphate
CAS No.	116271-22-8
Molecular Formula	C18H22N8O17P3
Molecular Weight	739.33 g/mol
Synonyms	PNP-GMP-PNP; 1-(2-Nitrophenyl)ethyl ester of guanosine 5'-(β,γ-imido)triphosphate; Guanylyl imidodiphosphate, 1-(2-nitrophenyl)ethyl ester; caged GTPγS analog; Non-hydrolyzable GTP analog; 2-Nitrophenylethyl-caged GTPγS; NPE-caged GTPγS; Photolabile GTPγS
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is characterized using advanced analytical techniques to guarantee identity, purity, and performance. Certificates of Analysis (COA) detailing purity, assay results, and impurity profiles are available upon request. We adhere to strict quality management protocols suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by UV spectrophotometry)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.