Description

8-((4-Bromo-2,3-Dioxobutyryl)Thio)Adenosine 5'Diphosphate is a chemically modified nucleotide analog designed for advanced biochemical research and development. This compound is a critical tool for studying and manipulating enzyme kinetics, particularly for ATP-dependent enzymes, due to its reactive bromodioxobutyryl-thio moiety. It is primarily utilized by research scientists and process developers in the pharmaceutical, biotechnology, and life science sectors for applications in enzymology, proteomics, and the development of novel diagnostic assays.

Application

• Enzyme Inhibition Studies: Acts as a potent, mechanism-based inhibitor for probing the active sites of kinases, ATPases, and other nucleotide-binding proteins.
• Affinity Labeling & Proteomics: Used as an affinity label to tag, isolate, and identify specific enzyme targets within complex biological mixtures.
• Biochemical Assay Development: Serves as a key reagent in the development of high-throughput screening (HTS) assays for drug discovery programs.
• Structural Biology: Aids in X-ray crystallography and NMR studies by covalently modifying enzymes to capture reaction intermediate states.
• Metabolic Pathway Research: Employed to investigate and modulate critical ATP-dependent pathways in cellular metabolism.
• Diagnostic Reagent Synthesis: Used as a building block for synthesizing specialized reagents in clinical diagnostics and biosensors.

Basic Information

Product Name	8-((4-Bromo-2,3-Dioxobutyryl)Thio)Adenosine 5'Diphosphate
CAS No.	115678-78-9
Molecular Formula	C14H17BrN5O13P2S
Molecular Weight	668.22 g/mol
Synonyms	8-[(4-Bromo-2,3-dioxobutyl)thio]adenosine 5'-(trihydrogen diphosphate); 8-BDB-TADP; 8-(4-Bromo-2,3-dioxobutylthio)adenosine diphosphate; Adenosine-5'-diphosphate, 8-[(4-bromo-2,3-dioxobutyl)thio]-; BDB-TADP; 8-(4-Bromo-2,3-dioxobutyrylthio)adenosine-5'-diphosphate
EINECS	Contact for details

Quality Control

Our production of 8-((4-Bromo-2,3-Dioxobutyryl)Thio)Adenosine 5'Diphosphate adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. All materials are characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.