Description

2'-o-Methyl-5-Methyluridine CAS NO 114952-97-5 is a chemically modified nucleoside derivative, serving as a critical building block in oligonucleotide synthesis. Its primary value lies in enhancing the stability and specificity of synthetic RNA and DNA analogs, which is essential for advanced therapeutic and diagnostic applications. This high-purity intermediate is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic research institutions focused on antisense oligonucleotides, siRNA, and mRNA technology development.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of modified RNA strands, improving nuclease resistance and target binding affinity.
• Therapeutic RNA Development: Used in the research and production of siRNA (small interfering RNA) and antisense oligonucleotides (ASOs) for gene silencing therapies.
• mRNA Vaccine & Therapeutic Platforms: Incorporated into synthetic mRNA constructs to modulate immunogenicity and enhance translational efficiency.
• Diagnostic Probe Design: Employed in the creation of highly specific molecular probes and primers for sensitive detection assays.
• Structural Biology Studies: Utilized to investigate RNA structure, function, and protein-RNA interactions.
• Biochemical Research: Serves as a substrate or inhibitor in enzymatic studies related to nucleoside metabolism and RNA processing.

Basic Information

Product Name	2'-o-Methyl-5-Methyluridine
CAS No.	114952-97-5
Molecular Formula	C11H16N2O6
Molecular Weight	272.25 g/mol
Synonyms	5-Methyl-2'-O-methyluridine; 2'-O-Methyl-5-methyluridine; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]-5-methylpyrimidine-2,4-dione; 2'-O-Methyl-5-methyl-Uridine; 5-Methyl-2'-O-methyl-U; 2'-OMe-5-MeU; 2'-O-Me-5-Me-uridine; Uridine, 2'-O-methyl-5-methyl-
EINECS	Contact for details

Quality Control

Our 2'-o-Methyl-5-Methyluridine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and MS, to ensure it meets the exacting standards required for pharmaceutical and biotech applications. A comprehensive Certificate of Analysis (COA) detailing purity, impurities, and residual solvents is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant and inert atmosphere conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.