Description

5-(3-Amino-1-Propyn-1-Yl)-2',3'-Dideoxy-5'-(Tetrahydrogen Triphosphate)Cytidine is a modified nucleoside triphosphate analog, a critical building block in nucleic acid chemistry and biochemistry. Its primary value lies in its role as a substrate for DNA polymerases, enabling the site-specific incorporation of modified bases into oligonucleotides. This compound is essential for researchers and manufacturers in pharmaceutical development, molecular diagnostics, and biotechnology, particularly for applications requiring labeled or functionalized nucleic acid probes.

Application

• Oligonucleotide Synthesis: Used as a modified triphosphate monomer for the enzymatic synthesis of DNA strands containing cytidine analogs.
• Next-Generation Sequencing (NGS): Acts as a specialized substrate in sequencing-by-synthesis platforms to study polymerase kinetics or introduce specific functionalities.
• Antiviral & Anticancer Drug Research: Serves as a key intermediate or active metabolite in the development of nucleoside-based therapeutic agents targeting viral replication or cellular proliferation.
• Molecular Probe Development: Enables the creation of fluorescently labeled or chemically tagged cytidine nucleotides for diagnostic assays and imaging.
• Biochemical Studies: Utilized in enzymatic assays to investigate the mechanism and fidelity of DNA and RNA polymerases.
• Aptamer and SELEX Technology: Incorporated into nucleic acid libraries to expand chemical diversity and select for high-affinity binding molecules.

Basic Information

Product Name	5-(3-Amino-1-Propyn-1-Yl)-2',3'-Dideoxy-5'-(Tetrahydrogen Triphosphate)Cytidine
CAS No.	114748-56-0
Molecular Formula	C₁₂H₁₉N₄O₁₂P₃
Molecular Weight	528.22 g/mol
Synonyms	5-(3-Aminoprop-1-yn-1-yl)-2',3'-dideoxycytidine-5'-triphosphate; 5-(3-Aminopropynyl)-2',3'-dideoxy-CTP; 5-Propargylamino-dideoxy-CTP; ddCTP-5-Propargylamine; Modified Cytidine Triphosphate; Aminoalkyne-modified dideoxyCTP; Nucleoside Triphosphate Analog; CAS 114748-56-0
EINECS	Contact for details

Quality Control

Every batch of our 5-(3-Amino-1-Propyn-1-Yl)-2',3'-Dideoxy-5'-(Tetrahydrogen Triphosphate)Cytidine is manufactured under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available for each lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Triphosphate Content (³¹P NMR)	≥ 85%
Biological Activity (Enzymatic Assay)	Suitable for polymerase incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.