Description

5'-o-Dmt-N2-Isobutyryl-2'-o-Methyl-D-Guanosine CAS NO 114745-26-5 is a high-purity, chemically modified nucleoside derivative, specifically a protected guanosine analog. This compound is a critical building block in the solid-phase synthesis of oligonucleotides, particularly for research and therapeutic applications requiring high-fidelity RNA sequences. It is essential for pharmaceutical R&D, biotechnology, and academic laboratories focused on antisense technology, siRNA, and mRNA synthesis.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated solid-phase synthesis of RNA and modified RNA strands.
• Therapeutic RNA Development: Used in the research and production of siRNA (small interfering RNA), antisense oligonucleotides (ASOs), and mRNA for therapeutic and vaccine applications.
• Structural Biology Studies: Employed to incorporate 2'-O-methyl modifications into RNA oligonucleotides for studying RNA structure, stability, and protein-RNA interactions.
• Diagnostic Probe Manufacturing: Serves as a raw material for synthesizing labeled probes and primers used in molecular diagnostics and next-generation sequencing (NGS).
• Biochemical Research: A vital reagent for studying RNA interference (RNAi) pathways and developing novel gene silencing tools.
• Process Development: Used in scaling up GMP-grade oligonucleotide manufacturing processes for clinical-stage and commercial therapeutics.

Basic Information

Product Name	5'-o-Dmt-N2-Isobutyryl-2'-o-Methyl-D-Guanosine
CAS No.	114745-26-5
Molecular Formula	C41H49N5O9
Molecular Weight	755.87 g/mol
Synonyms	2'-O-Methyl-5'-O-(4,4'-dimethoxytrityl)-N2-isobutyrylguanosine; DMT-G(ib)-2'-OMe; 5'-O-DMT-2'-O-methyl-N2-isobutyryl-guanosine; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-methyl-N2-(2-methyl-1-oxopropyl)-; N2-Isobutyryl-2'-O-methyl-5'-O-(4,4'-dimethoxytrityl)guanosine; 2'-O-Methyl-N2-isobutyryl-5'-O-(4,4'-dimethoxytrityl)guanosine; Protected 2'-O-Methylguanosine Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

Our 5'-o-Dmt-N2-Isobutyryl-2'-o-Methyl-D-Guanosine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the stringent requirements for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and compliance with research and cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. Use under an inert atmosphere (argon or nitrogen) for critical applications.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.