Description

Formycin A 5'-(Betagamma-Imido)Triphosphate is a non-hydrolyzable ATP analog, specifically designed to inhibit ATP-dependent enzymes and processes. This compound is critical for biochemical research, serving as a potent and specific inhibitor of ATPases, kinases, and other nucleotide-binding proteins. It is an essential tool for researchers in enzymology, molecular biology, and signal transduction studies who require a stable, hydrolysis-resistant ligand to probe ATP-binding sites and elucidate metabolic pathways.

Application

• Enzyme Inhibition Studies: Used as a potent, non-hydrolyzable inhibitor for ATP-dependent enzymes such as ATPases, kinases, and helicases.
• Biochemical Assay Development: Serves as a critical component in assays designed to measure enzyme activity, ligand binding, and signal transduction pathways.
• Structural Biology Research: Employed in X-ray crystallography and NMR studies to lock target proteins in specific conformational states for structural analysis.
• Cellular Metabolism Research: A key tool for investigating energy metabolism, mitochondrial function, and nucleotide signaling within cells.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify novel inhibitors or modulators of ATP-binding targets.
• Academic & Pharmaceutical R&D: Fundamental research reagent for universities, research institutes, and biopharmaceutical companies.

Basic Information

Product Name	Formycin A 5'-(Betagamma-Imido)Triphosphate
CAS No.	114661-04-0
Molecular Formula	C16H22N6O13P3
Molecular Weight	623.29 g/mol
Synonyms	Formycin A 5'-(β,γ-Imido)triphosphate; Adenosine 5'-(β,γ-imido)triphosphate Formycin A analog; App(NH)p-Formycin A; β,γ-Imido-ATP (Formycin A); 5'-Adenylyl (β,γ-methylenediphosphonate) Formycin A; Formycin A AppNHp; Non-hydrolyzable ATP analog (Formycin A); Formycin A 5'-(β,γ-Methylene)triphosphate (common misnomer)
EINECS	Contact for details

Quality Control

Every batch of Formycin A 5'-(Betagamma-Imido)Triphosphate is manufactured and tested under stringent quality management protocols to ensure the highest standards of purity and consistency for research applications. Our products undergo rigorous analytical characterization, including HPLC, NMR, and MS, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your research integrity and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry atmosphere before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by weight)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.