Description

3'-Deoxy-5'-o-(Dimethoxytrityl)-5-Methyluridine is a key protected nucleoside derivative, essential for the synthesis of oligonucleotides and modified nucleic acids. This compound is critical for ensuring high coupling efficiency and purity in automated solid-phase synthesis, directly impacting the yield and performance of the final oligonucleotide product. It is primarily required by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors engaged in antisense therapy, siRNA development, and molecular probe production.

Application

• Oligonucleotide Synthesis: Serves as a crucial phosphoramidite building block in the automated solid-phase synthesis of DNA and RNA strands.
• Antisense & siRNA Therapeutics: Used in the production of therapeutic oligonucleotides for gene silencing and targeted drug development.
• Molecular Diagnostics: Enables the creation of specialized probes and primers for PCR, sequencing, and other diagnostic assays.
• Research & Development: A vital reagent in academic and industrial labs for studying nucleic acid chemistry and developing novel bioconjugates.
• API Intermediate: Functions as a high-purity intermediate in the synthesis of active pharmaceutical ingredients (APIs) for nucleotide-based drugs.

Basic Information

Product Name	3'-Deoxy-5'-o-(Dimethoxytrityl)-5-Methyluridine
CAS No.	114551-15-4
Molecular Formula	C31H32N2O7
Molecular Weight	544.60 g/mol
Synonyms	5-Methyl-2'-deoxyuridine 5'-(4,4'-dimethoxytrityl) ether; 3'-Deoxy-5'-O-DMT-5-methyluridine; 5'-O-(4,4'-Dimethoxytrityl)-3'-deoxy-5-methyluridine; DMT-3'-deoxy-5-methyluridine; 3'-dT DMT; 5-Methyl-3'-deoxyuridine DMT ether; 3'-Deoxythymidine 5'-O-DMT; 114551-15-4
EINECS	Contact for details

Quality Control

Our 3'-Deoxy-5'-o-(Dimethoxytrityl)-5-Methyluridine is manufactured under strict quality systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles as determined by advanced analytical techniques including HPLC, NMR, and mass spectrometry. We support compliance with cGMP and ICH guidelines for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Individual impurity ≤ 0.5% Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.