Description

Aprotinin, Arg(15)-Glu(52)- CAS NO 114264-91-4 is a specific, modified variant of the serine protease inhibitor aprotinin, characterized by the substitution of arginine at position 15 and glutamic acid at position 52. This engineered peptide is valued for its enhanced or altered inhibitory profile against specific proteolytic enzymes like trypsin, plasmin, and kallikrein, offering researchers a precise tool for mechanistic studies. It is primarily utilized in advanced biochemical research, pharmaceutical development for investigating protease pathways, and in the production of high-purity diagnostic reagents.

Application

• Biochemical Research: A key reagent for studying the kinetics, specificity, and inhibition mechanisms of serine proteases in controlled laboratory settings.
• Pharmaceutical Development: Used in drug discovery programs targeting protease-related diseases, serving as a reference compound or a tool for target validation.
• Diagnostic Manufacturing: Incorporated into the formulation of specialized diagnostic kits and assays that require precise protease inhibition for accurate results.
• Cell Culture & Tissue Engineering: Applied in research media to inhibit proteolytic activity, thereby protecting cells and sensitive proteins during experimental procedures.
• Enzyme Purification & Characterization: Employed in affinity chromatography and activity assays for the isolation and functional analysis of specific proteases.

Basic Information

Product Name	Aprotinin, Arg(15)-Glu(52)- CAS NO 114264-91-4
CAS No.	114264-91-4
Molecular Formula	C284H432N84O79S7
Molecular Weight	Approx. 6512.0 g/mol
Synonyms	Arg15-Glu52-Aprotinin; Aprotinin variant; BPTI variant (Arg15, Glu52); Basic Pancreatic Trypsin Inhibitor variant; Kunitz protease inhibitor variant; Trypsin Inhibitor, Basic Pancreatic, Arg15-Glu52; TLCK-Aprotinin analog; CAS 114264-91-4
EINECS	Contact for details

Quality Control

Every batch of our Aprotinin, Arg(15)-Glu(52)- is produced and tested under stringent quality management systems. We ensure compliance with high-purity standards suitable for research and development applications. Comprehensive analytical testing, including HPLC for purity and peptide mapping for sequence confirmation, is performed. A Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (Enzymatic Activity)	≥ 90% of stated inhibitory activity
Water Content (KF)	≤ 5.0%
Peptide Content (UV)	≥ 80.0%
Endotoxin	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.