Description

5'-o-(Dimethoxytrityl)-5,6-Dihydrothymidine is a key nucleoside derivative, specifically a protected dihydrothymidine analog, widely used as a critical building block in oligonucleotide synthesis. Its primary value lies in enabling the controlled assembly of DNA and RNA sequences for research and therapeutic development. This high-purity intermediate is essential for pharmaceutical companies, biotechnology research institutes, and diagnostic manufacturers engaged in antisense therapy, siRNA production, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: A vital phosphoramidite precursor for the solid-phase synthesis of DNA and RNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for therapeutic applications.
• siRNA and miRNA Manufacturing: Serves as a modified nucleoside component in the synthesis of small interfering RNA (siRNA) and microRNA (miRNA) for gene silencing studies.
• Diagnostic Probe Synthesis: Employed in creating labeled probes for genetic testing, fluorescence in situ hybridization (FISH), and PCR applications.
• Research & Development: A fundamental reagent in academic and industrial labs for studying nucleic acid chemistry, stability, and function.
• Pharmaceutical Intermediates: Acts as a high-value intermediate in the cGMP-compliant production of novel nucleic acid-based therapeutics.

Basic Information

Item	Details
Product Name	5'-o-(Dimethoxytrityl)-5,6-Dihydrothymidine
CAS No.	113974-50-8
Molecular Formula	C33H36N2O7
Molecular Weight	572.65 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-5,6-dihydrothymidine; 5'-O-DMT-5,6-dihydrothymidine; DMT-Dihydrothymidine; 1-[(2R,4S,5R)-4-Hydroxy-5-[(hydroxy{4-[(4-methoxyphenyl)phenylmethoxy]phenyl})methyl]oxolan-2-yl]-5-methylpyrimidine-2,4-dione; 5,6-Dihydro-5'-O-(4,4'-dimethoxytrityl)thymidine; Dihydrothymidine DMT ether
EINECS	Contact for details

Quality Control

Our 5'-o-(Dimethoxytrityl)-5,6-Dihydrothymidine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to stringent in-process controls, and our quality commitment supports development under GMP guidelines for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under a dry inert atmosphere or in a controlled environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Single Unknown Impurity	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.