Description

N4-Acetyl-2'-o-Methyl-Cytidine is a chemically modified nucleoside derivative, specifically an acetylated and methylated analog of cytidine. This compound is of significant value in oligonucleotide synthesis and pharmaceutical research, where it serves as a critical building block for creating modified RNA strands with enhanced stability and specific biological properties. It is primarily needed by research institutions, biotechnology companies, and pharmaceutical developers working on antisense oligonucleotides, siRNA therapeutics, and diagnostic probes.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of modified RNA sequences.
• Therapeutic RNA Development: Used in the research and production of siRNA (small interfering RNA) and antisense oligonucleotides to improve nuclease resistance and pharmacokinetic profiles.
• Structural Biology Studies: Employed to investigate RNA structure, function, and protein-RNA interactions by introducing specific modifications.
• Diagnostic Probe Fabrication: Incorporated into labeled probes for molecular diagnostics and hybridization assays to enhance specificity and stability.
• Bioconjugation & Labeling: Serves as a starting point for further chemical modifications and attachment of reporter molecules like fluorophores.

Basic Information

Product Name	N4-Acetyl-2'-o-Methyl-Cytidine
CAS No.	113886-71-8
Molecular Formula	C13H19N3O6
Molecular Weight	313.31 g/mol
Synonyms	2'-O-Methyl-N4-acetylcytidine; 1-((2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl)-4-(acetylamino)pyrimidin-2-one; N4-Acetyl-2'-O-methylcytidine; 2'-O-Me-N4-Ac-cytidine; 2'-O-Methyl-N4-acetylcytidine; Acetylated 2'-O-methylcytidine; 2'-OMe-N4-Ac-C
EINECS	Contact for details

Quality Control

Our N4-Acetyl-2'-o-Methyl-Cytidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and MS, to ensure it meets the high standards required for pharmaceutical research and oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions. For long-term storage, consider desiccation and an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.