Description

2'-(o-Methyl)-5-Methylcytidine is a chemically modified nucleoside analog, specifically a derivative of cytidine with methylation at the 2'-O and 5 positions. This modification is crucial for enhancing the stability and biological activity of oligonucleotides, making it a valuable building block in advanced therapeutic research. It is primarily utilized by pharmaceutical R&D teams and biotechnology companies engaged in the development of novel antisense oligonucleotides, siRNA, and other RNA-based therapeutics where nuclease resistance and precise pharmacokinetic tuning are required.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A key phosphoramidite monomer for constructing oligonucleotides with improved metabolic stability and binding affinity.
• siRNA and miRNA Therapeutics: Used to introduce specific 2'-O-methyl modifications into RNA strands to reduce immunogenicity and enhance in vivo efficacy.
• Structural Biology Studies: Employed to probe RNA structure-function relationships and enzyme-substrate interactions.
• Diagnostic Probe Development: Serves as a modified base in the design of highly specific and stable hybridization probes for diagnostic assays.
• Medicinal Chemistry & Drug Discovery: A vital intermediate in the research and development of nucleoside-based antiviral and anticancer agents.

Basic Information

Product Name	2'-(o-Methyl)-5-Methylcytidine
CAS No.	113886-70-7
Molecular Formula	C12H19N3O5
Molecular Weight	285.30 g/mol
Synonyms	5-Methyl-2'-O-methylcytidine; 2'-O-Methyl-5-methylcytidine; 2'-OMe-5-Me-Cytidine; 1-((2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl)-5-methylpyrimidine-2,4-dione; N4-Acetyl-2'-O-methyl-5-methylcytidine (precursor); 2'-O-Methyl-5-methylcytidine (RNA Grade)
EINECS	Contact for details

Quality Control

Our 2'-(o-Methyl)-5-Methylcytidine is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and development applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by NMR and Mass Spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.