Description

5'-O-[Bis(4-Methoxyphenyl)Phenylmethyl]Cytidine is a critical nucleoside derivative used as a key protecting group reagent in oligonucleotide synthesis. This compound matters because it enables the precise and efficient construction of DNA and RNA sequences by selectively protecting the 5'-hydroxyl group of cytidine, ensuring high coupling yields and purity in the final product. It is an essential raw material for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries engaged in antisense therapy, gene synthesis, and molecular biology applications.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a 5'-OH protecting group reagent for cytidine phosphoramidites in automated DNA/RNA synthesizers.
• Antisense Oligonucleotide (ASO) Production: A key building block in the manufacture of therapeutic oligonucleotides for targeting specific mRNA sequences.
• Research-Grade Primer and Probe Synthesis: Used in academic and industrial labs to synthesize custom primers, probes, and gene fragments for PCR, sequencing, and hybridization.
• siRNA and miRNA Synthesis: Critical for the production of short interfering RNA (siRNA) and microRNA (miRNA) molecules used in gene silencing and functional genomics studies.
• Diagnostic Kit Manufacturing: A component in the production of labeled probes and capture sequences for in vitro diagnostic (IVD) assays and kits.
• Scale-Up GMP Manufacturing: Serves as a starting material for the synthesis of cytidine phosphoramidites under Good Manufacturing Practice (GMP) conditions for clinical-grade oligonucleotides.

Basic Information

Product Name	5'-O-[Bis(4-Methoxyphenyl)Phenylmethyl]Cytidine
CAS No.	112897-99-1
Molecular Formula	C31H31N3O7
Molecular Weight	557.60 g/mol
Synonyms	5'-O-DMT-Cytidine; 5'-O-(4,4'-Dimethoxytrityl)cytidine; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine (common protected form); DMT-Cytidine; 1-[(2R,3R,4R,5R)-5-[Bis(4-methoxyphenyl)-phenylmethoxy]-3,4-dihydroxyoxolan-2-yl]-4-aminopyrimidin-2-one; Cytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; 5'-O-(4,4'-Dimethoxytriphenylmethyl)cytidine
EINECS	Contact for details

Quality Control

Our 5'-O-[Bis(4-Methoxyphenyl)Phenylmethyl]Cytidine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (typically ≥98.0%), to ensure it meets the stringent requirements for oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) with detailed chromatographic data, and our quality systems support compliance with relevant guidelines for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccated conditions at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.