Description

5'-O-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-7-Deaza-2'-Deoxyguanosine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in the automated synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of modified 7-deaza-2'-deoxyguanosine analogs into synthetic DNA strands, which is essential for advanced research and therapeutic development. This compound is indispensable for biotechnology, pharmaceutical R&D, and diagnostic companies focused on antisense technology, PCR primers, probes, and the development of novel nucleic acid-based therapeutics.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer in solid-phase automated DNA synthesizers for the site-specific introduction of 7-deaza-guanosine modifications.
• Antisense & siRNA Therapeutics: Used in the production of modified oligonucleotides with enhanced nuclease resistance, binding affinity, and pharmacokinetic properties for therapeutic applications.
• PCR & Molecular Diagnostics: Manufacture of specialized primers and probes containing 7-deazaguanine to reduce secondary structure and improve amplification efficiency, particularly for GC-rich targets.
• Structural Biology Studies: Synthesis of DNA strands for studying nucleic acid structure, stability, and protein-DNA interactions where standard guanine residues are problematic.
• Next-Generation Sequencing (NGS): Production of custom oligonucleotide libraries and adapters with specific modifications to improve sequencing performance.
• Basic Biochemical Research: A vital reagent for academic and industrial research exploring DNA function, repair mechanisms, and the development of novel bioassays.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-7-Deaza-2'-Deoxyguanosine
CAS No.	111869-42-2
Molecular Formula	C37H41N5O6
Molecular Weight	651.76 g/mol
Synonyms	5'-O-DMT-N2-(dimethylaminomethylene)-7-deaza-2'-deoxyguanosine; N2-(Dimethylaminomethylidene)-5'-O-(4,4'-dimethoxytrityl)-7-deaza-2'-deoxyguanosine; DMT-dG(7-deaza)amidite precursor; 7-Deaza-2'-deoxyguanosine, N2-[(dimethylamino)methylene]-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; Protected 7-Deaza-2'-deoxyguanosine; c7dG(DMF) DMT
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with the high-purity standards required for oligonucleotide synthesis. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing lot-specific results are available upon request. We adhere to a quality management system to ensure batch-to-batch consistency and reliability for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.