Description

Uridine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-5-Ethynyl is a chemically modified nucleotide triphosphate analog, specifically engineered for advanced biochemical research and diagnostic applications. This compound is a critical reagent for studying nucleic acid metabolism and for the development of novel molecular probes and therapeutic agents. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, medicinal chemistry, and diagnostic assay development.

Application

• Nucleic Acid Research: As a substrate analog for studying DNA and RNA polymerase activity, chain termination, and enzymatic mechanisms.
• Click Chemistry Labeling: The 5-ethynyl group enables efficient, site-specific conjugation with azide-containing dyes or tags via copper-catalyzed azide-alkyne cycloaddition (CuAAC) for nucleic acid labeling and detection.
• Antiviral Drug Development: Serves as a potential precursor or active metabolite in the research of nucleoside analog-based antiviral therapies.
• Diagnostic Probe Synthesis: Used in the manufacture of labeled nucleotides for next-generation sequencing (NGS), microarray fabrication, and in situ hybridization assays.
• Biochemical Assays: Acts as a high-energy phosphate donor or a specific inhibitor in enzymatic studies and high-throughput screening platforms.
• Medicinal Chemistry: A key building block for the synthesis of novel nucleoside triphosphate analogs with potential therapeutic applications.

Basic Information

Product Name	Uridine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-5-Ethynyl
CAS No.	111289-87-3
Molecular Formula	C₁₂H₁₆N₂O₁₄P₃
Molecular Weight	529.18 g/mol
Synonyms	2'-Deoxy-5-ethynyluridine 5'-triphosphate; 5-Ethynyl-2'-deoxyuridine 5'-triphosphate; dEUTP; EdUTP; 5-Ethynyl-dUTP; 2'-Deoxy-5-ethynyl-UTP; 5-Ethynyl-2'-deoxyuridine triphosphate
EINECS	Contact for details

Quality Control

Our Uridine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-5-Ethynyl is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via mass spectrometry, to ensure it meets the exacting standards required for sensitive research applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated and under an inert atmosphere to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.