Description

2',2'-Difluorodeoxycytidine 5'-Triphosphate is a modified nucleoside triphosphate analog, a critical building block for nucleic acid synthesis. This compound is essential for applications requiring the incorporation of cytidine analogs into DNA or RNA strands, offering enhanced stability and specific functionality. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, diagnostics, and pharmaceutical development, particularly for next-generation sequencing, PCR applications, and the synthesis of oligonucleotide therapeutics.

Application

• Next-Generation Sequencing (NGS): Used as a substrate for DNA polymerases in sequencing-by-synthesis platforms to enable accurate base calling.
• Polymerase Chain Reaction (PCR): Employed in specialized PCR applications, including mutagenesis and the synthesis of modified DNA probes.
• Oligonucleotide Synthesis: A key raw material for the solid-phase synthesis of therapeutic and diagnostic oligonucleotides containing modified cytidine residues.
• Enzymatic DNA/RNA Synthesis: Serves as a high-purity nucleotide triphosphate (NTP) for template-independent enzymatic synthesis methods.
• Biochemical Research: Used in studies of DNA polymerase kinetics, fidelity, and mechanism with fluorinated nucleotide analogs.
• Antiviral & Anticancer Drug Development: Investigated as a potential metabolite or prodrug component in the development of nucleoside-based therapies.

Basic Information

Product Name	2',2'-Difluorodeoxycytidine 5'-Triphosphate
CAS No.	110988-86-8
Molecular Formula	C9H14F2N3O13P3
Molecular Weight	545.13 g/mol
Synonyms	2'-Deoxy-2',2'-difluorocytidine 5'-triphosphate; dFdCTP; Gemcitabine triphosphate; 2',2'-Difluoro-2'-deoxycytidine 5'-triphosphate; 5'-Triphosphoric acid, 2'-deoxy-2',2'-difluorocytidine; [(2R,3S,5R)-5-(4-Amino-2-oxo-1,2-dihydropyrimidin-1-yl)-3-fluoro-4-fluoro-2-(hydroxymethyl)oxolan-2-yl]methyl tetrahydrogen triphosphate
EINECS	Contact for details

Quality Control

Our 2',2'-Difluorodeoxycytidine 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Triphosphate Content	≥ 85%
Endotoxin	< 10 EU/mg
Biological Activity	Tested and suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.