Description

Cytidine, n-Benzoyl-5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-o-Methyl- is a sophisticated, protected nucleoside derivative designed for advanced synthetic chemistry. This compound is a critical building block in the synthesis of oligonucleotides, enabling the precise construction of RNA analogs and therapeutic nucleic acids. It is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors, particularly those developing antisense oligonucleotides, siRNA, and mRNA-based therapeutics.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of modified RNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for targeted gene silencing therapies.
• siRNA and miRNA Synthesis: Enables the incorporation of specific 2'-O-methyl modifications to enhance stability and efficacy of RNA interference agents.
• mRNA Therapeutic Research: Serves as a modified nucleoside triphosphate (NTP) precursor for in vitro transcription to produce stabilized mRNA.
• Chemical Biology Probes: Utilized in the creation of labeled or tagged nucleosides for studying RNA structure, function, and metabolism.
• Pharmaceutical Intermediate: Acts as a high-value intermediate in the multi-step synthesis of complex nucleoside-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	Cytidine, n-Benzoyl-5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-o-Methyl-
CAS No.	110764-74-4
Molecular Formula	C38H36N3O8
Molecular Weight	662.71 g/mol
Synonyms	5'-O-DMT-2'-O-methyl-N-benzoylcytidine; 2'-O-Methyl-5'-O-(4,4'-dimethoxytrityl)-N-benzoylcytidine; N4-Benzoyl-2'-O-methyl-5'-O-(4,4'-dimethoxytrityl)cytidine; DMT-2'-O-Me-C(Bz) Amidite precursor; 2'-O-Methyl-N-benzoyl-5'-O-DMT-cytidine; 2'-O-Methylcytidine, N-benzoyl-5'-O-DMT protected
EINECS	Contact for details

Quality Control

This high-purity building block is manufactured under controlled GMP-like conditions to ensure batch-to-batch consistency for sensitive synthetic applications. Quality is verified through a comprehensive suite of analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each batch, confirming identity, purity, and absence of critical impurities.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.