Description

N6-Benzoyl-5'-(Dimethoxytrityl)-2'-o-Methyladenosine is a key protected nucleoside phosphoramidite building block used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity during the synthesis of modified RNA strands. It is primarily required by research institutions, diagnostic developers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, and other therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: As a 2'-O-methyl modified adenosine phosphoramidite precursor for solid-phase synthesis.
• Therapeutic RNA Development: Building block for creating nuclease-resistant antisense oligonucleotides and siRNA therapeutics.
• Diagnostic Probe Manufacture: Used in the synthesis of labeled probes for FISH, microarrays, and other molecular diagnostic assays.
• Basic Biochemical Research: For studying RNA structure, function, and protein-RNA interactions using chemically modified oligonucleotides.
• Aptamer Discovery: Enables the generation of SELEX libraries containing 2'-O-methyl modified nucleotides to enhance aptamer stability.

Basic Information

Product Name	N6-Benzoyl-5'-(Dimethoxytrityl)-2'-o-Methyladenosine
CAS No.	110764-72-2
Molecular Formula	C39H37N5O7
Molecular Weight	687.75 g/mol
Synonyms	2'-O-Methyl-N6-benzoyl-5'-O-(4,4'-dimethoxytrityl)adenosine; 2'-O-Me-5'-DMT-N6-Bz-Adenosine; 5'-O-DMT-2'-O-methyl-N6-benzoyladenosine; 2'-O-Methyladenosine, N6-benzoyl-5'-O-(4,4'-dimethoxytrityl)-; DMT-2'-O-Me-N6-Bz-A; N6-Bz-2'-O-Me-5'-DMT-Adenosine; Protected 2'-O-Methyladenosine Phosphoramidite Intermediate
EINECS	Contact for details

Quality Control

Our production of this high-value nucleic acid building block adheres to stringent in-process controls to ensure batch-to-batch consistency and high purity, critical for successful oligonucleotide synthesis. Quality is verified via HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content is provided with each batch to support your quality and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under anhydrous conditions. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.