Description

5'-Dimethoxytrityl-N2-Phenoxyacetyl-2'-Deoxyguanosine-3'-(2-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA strands for research, diagnostics, and therapeutic applications. It is a fundamental building block for biotechnology, pharmaceutical R&D, and molecular biology laboratories engaged in synthetic genomics and antisense technology development.

Application

• Oligonucleotide Synthesis: Primary monomer for the automated, solid-phase synthesis of DNA sequences, including primers, probes, and gene fragments.
• Therapeutic Oligonucleotide Manufacturing: Key raw material in the production of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Production: Used to synthesize labeled or unlabeled DNA probes for PCR, qPCR, microarrays, and in-situ hybridization.
• Synthetic Biology & Genomics: Essential for gene synthesis, assembly, and the construction of synthetic DNA libraries for research and development.
• Antisense Research: Building block for creating modified oligonucleotides used in gene function studies and target validation.
• CRISPR Guide RNA Synthesis: Employed in the synthesis of DNA templates for in vitro transcription of guide RNAs (gRNAs).

Basic Information

Product Name	5'-Dimethoxytrityl-N2-Phenoxyacetyl-2'-Deoxyguanosine-3'-(2-Cyanoethyl-N,n-Diisopropyl)Phosphoramidite
CAS No.	110522-83-3
Molecular Formula	C47H53N6O8P
Molecular Weight	860.94 g/mol
Synonyms	dG(Pac) Phosphoramidite; 5'-DMT-2'-dG(N-Pac)-3'-CE Phosphoramidite; N-Phenoxyacetyl-2'-deoxyguanosine Phosphoramidite; Guanosine, 2'-deoxy-N-(phenoxyacetyl)-, 5'-[bis(4-methoxyphenyl)phenylmethyl] 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Pac-dG Phosphoramidite; dG Pac-CE Phosphoramidite; 2'-Deoxyguanosine, N2-protected phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict quality management systems to ensure exceptional purity and batch-to-batch consistency, critical for successful oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) detailing purity (HPLC), identity (NMR, MS), moisture content (KF), and other critical parameters. Our quality commitment aligns with the standards required for GMP-grade manufacturing of therapeutic oligonucleotides.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.