Description

5'-o-Dmtr-N6-Fmoc-Da-Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the automated production of DNA and RNA strands. It is primarily required by biotechnology, pharmaceutical, and diagnostic companies engaged in research, therapeutic development, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: As a key building block in automated solid-phase synthesizers for the production of custom DNA and RNA sequences.
• Therapeutic Development: For the synthesis of antisense oligonucleotides (ASOs), siRNA, and aptamers used in novel drug modalities.
• Research Reagents: In academic and industrial laboratories for creating probes, primers, and gene fragments for PCR, sequencing, and cloning.
• Diagnostic Kits: Manufacturing components for molecular diagnostic assays, including probes for qPCR and next-generation sequencing.
• CRISPR/Cas9 Systems: Synthesis of guide RNAs (gRNAs) and donor DNA templates for gene editing applications.

Basic Information

Product Name	5'-o-Dmtr-N6-Fmoc-Da-Phosphoramidite
CAS No.	109420-86-2
Molecular Formula	C47H51N6O8P
Molecular Weight	858.93 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-[(9H-fluoren-9-ylmethoxy)carbonyl]-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; N6-Fmoc-5'-O-DMTr-2'-deoxyadenosine-3'-phosphoramidite; Fmoc-dA Phosphoramidite; 2'-Deoxyadenosine, N6-[(9H-fluoren-9-ylmethoxy)carbonyl]-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; DA(Fmoc) Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-o-Dmtr-N6-Fmoc-Da-Phosphoramidite is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch undergoes comprehensive analytical testing, including HPLC for purity and 31P/1H NMR for structural confirmation, to ensure high coupling efficiency and batch-to-batch consistency. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and moisture-sensitive nature, the material should be brought to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.