Description

7,25-Dihydroxycholesterol is a key oxysterol metabolite involved in the cholesterol biosynthesis and signaling pathways. This compound is of significant interest for research into lipid metabolism, liver X receptor (LXR) activation, and bile acid synthesis. It serves as a critical biochemical standard and research tool for pharmaceutical R&D, academic institutions, and diagnostic laboratories focused on metabolic disorders and cardiovascular disease.

Application

• Biochemical Research Standard: Used as a high-purity reference standard for analytical method development and validation in lipidomics and metabolomics studies.
• Pharmaceutical R&D: Investigated as a precursor or modulator in drug discovery programs targeting nuclear receptors like LXRs for treating atherosclerosis and metabolic diseases.
• Cell Biology Studies: Applied in cell culture experiments to study the effects of specific oxysterols on cholesterol homeostasis, gene expression, and cell signaling pathways.
• Diagnostic Development: Utilized as a calibrant or control in the development of clinical assays (e.g., LC-MS/MS) for quantifying oxysterol levels in biological samples.
• Enzymology: Serves as a substrate for studying the activity and kinetics of enzymes like cytochrome P450s involved in cholesterol hydroxylation.

Basic Information

Item	Detail
Product Name	7,25-Dihydroxycholesterol
CAS No.	107655-65-2
Molecular Formula	C27H46O3
Molecular Weight	418.66 g/mol
Synonyms	(25R)-26-Hydroxycholest-5-ene-3β,7α-diol; 7α,25-Dihydroxycholesterol; 7α,26-Dihydroxycholesterol; 3β,7α-Dihydroxy-5-cholesten-26-ol; 5-Cholestene-3β,7α,26-triol; Cholest-5-ene-3β,7α,26-triol
EINECS	Contact for details

Quality Control

Our 7,25-Dihydroxycholesterol is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for research and development. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability and consistent quality.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere. Allow the product to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.