Description

Methotrexate 5-Aminoallyl-2'-Deoxyuridine 5'-Monophosphate is a chemically modified nucleotide analog designed for advanced research and diagnostic applications. This compound is particularly valuable for its role as a key intermediate in the synthesis of labeled probes and as a tool for studying enzyme mechanisms and nucleic acid interactions. It is primarily sought by researchers and developers in the fields of pharmaceutical development, molecular biology, and diagnostic assay manufacturing. The product is supplied with a high level of purity and batch-to-batch consistency to ensure reliable experimental results.

Application

• Biochemical Research: Used as a specialized substrate or inhibitor in enzymatic studies, particularly those involving thymidylate synthase or other folate-dependent enzymes.
• Probe Synthesis: Serves as a critical precursor for the chemical synthesis of fluorescently or radioactively labeled nucleotide probes for hybridization assays.
• Antimetabolite Studies: Employed in cancer research to investigate the mechanisms of action and resistance pathways of antifolate drugs like methotrexate.
• Diagnostic Development: Acts as a key component in the development of in vitro diagnostic (IVD) kits and assays targeting specific metabolic pathways.
• Pharmaceutical Intermediates: Utilized as a building block in the research and development of novel chemotherapeutic agents and targeted therapies.

Basic Information

Product Name	Methotrexate 5-Aminoallyl-2'-Deoxyuridine 5'-Monophosphate
CAS No.	106449-21-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5-Aminoallyl-dUMP-Methotrexate Conjugate; MTX-5-aa-dUMP; Methotrexate-linked 5-Aminoallyl-2'-deoxyuridine monophosphate; 5-(3-Aminoallyl)-2'-deoxyuridine 5'-monophosphate methotrexate derivative; Nucleoside-Methotrexate Conjugate; Antifolate-nucleotide hybrid; Research Compound 106449-21-2
EINECS	Contact for details

Quality Control

Every batch of our Methotrexate 5-Aminoallyl-2'-Deoxyuridine 5'-Monophosphate is manufactured and tested under a strict quality management system. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to verify chemical identity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with each shipment to ensure full traceability and compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.