Description

2'-Azido-2'-Deoxyuridine-5'-Triphosphate is a chemically modified nucleotide triphosphate analog, a critical reagent for advanced molecular biology and biochemical research. Its value lies in its ability to act as a chain terminator and click chemistry handle, enabling precise labeling and sequencing applications. This compound is essential for researchers and manufacturers in next-generation sequencing (NGS), nucleic acid synthesis, and bioconjugation workflows.

Application

• Next-Generation Sequencing (NGS): Used as a reversible terminator in sequencing-by-synthesis platforms for high-throughput DNA sequencing.
• DNA Synthesis & Labeling: Acts as a substrate for DNA polymerases to incorporate azide-modified nucleotides for subsequent bioorthogonal click chemistry conjugation (e.g., with alkynes).
• Bioconjugation & Probe Development: Enables the site-specific labeling of nucleic acids with fluorophores, biotin, or other functional groups via azide-alkyne cycloaddition.
• Enzymatic Studies: Serves as a tool to study polymerase kinetics, fidelity, and mechanism of action with modified substrates.
• Antiviral Research: Investigated for its potential as a prodrug or chain terminator in the study of viral replication mechanisms.
• Diagnostic Assay Development: Utilized in the creation of sensitive and specific molecular diagnostic tests and biosensors.

Basic Information

Product Name	2'-Azido-2'-Deoxyuridine-5'-Triphosphate
CAS No.	106448-30-0
Molecular Formula	C9H13N6O14P3
Molecular Weight	546.13 g/mol
Synonyms	2'-Azido-2'-deoxyuridine 5'-triphosphate; 2'-N3-dUTP; Azido-dUTP; 2'-Azido-2'-deoxyuridine-5'-triphosphate trisodium salt; 2'-Azido-dUTP; 5'-Triphosphoric acid 2'-azido-2'-deoxyuridine; 2'-Azido-2'-deoxy-Uridine-5'-triphosphate; AZTTP analog
EINECS	Contact for details

Quality Control

Our 2'-Azido-2'-Deoxyuridine-5'-Triphosphate is manufactured under stringent quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards required for molecular biology reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated at -80°C. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity	Suitable for use as a polymerase substrate
Endotoxin Level	< 1.0 EU/mg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.