Description

5'-O-(4,4'-Dimethoxytrityl)-5-Methyl-N4-Benzoyl-2'-Deoxycytidine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of 5-methylcytosine, a critical epigenetic modification, into synthetic DNA strands. This compound is a fundamental building block for research institutions, diagnostic developers, and pharmaceutical companies engaged in therapeutic oligonucleotide development, PCR probe synthesis, and advanced genomic research.

Application

• Therapeutic Oligonucleotide Synthesis: A critical raw material for manufacturing antisense oligonucleotides (ASOs), siRNA, and aptamers targeting genetic diseases.
• PCR & Molecular Diagnostics: Used in the production of specialized probes and primers where cytosine methylation status needs to be controlled or analyzed.
• Gene Synthesis & Cloning: Enables the construction of synthetic genes with site-specific 5-methylcytosine modifications for functional studies.
• Epigenetics Research: Essential for creating custom oligonucleotide tools to study DNA methylation patterns and their biological impacts.
• Next-Generation Sequencing (NGS): Used in the preparation of sequencing libraries and probes that require methylation-aware design.
• Antisense Technology: Key building block for creating stable, nuclease-resistant oligonucleotides for research and therapeutic applications.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-5-Methyl-N4-Benzoyl-2'-Deoxycytidine-3'-(2-Cyanoethyl-N,N-Diisopropyl)Phosphoramidite
CAS No.	105931-57-5
Molecular Formula	C47H54N5O8P
Molecular Weight	848.0 g/mol
Synonyms	5-Methyl-dC(Bz) Phosphoramidite; dmf-dC(Bz) Phosphoramidite; 5-Methyl-2'-deoxycytidine, N4-benzoyl-, 5'-(bis(4-methoxyphenyl)phenylmethyl) 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 5-Me-dC Phosphoramidite; 5-Methylcytosine Phosphoramidite (Bz protected); dCMe Phosphoramidite; Amidite for 5-methylcytosine
EINECS	Contact for details

Quality Control

Our 5-methyl-dC phosphoramidite is manufactured under strict quality systems suitable for GMP-grade oligonucleotide production. Each batch undergoes comprehensive analytical testing, including HPLC purity assessment, 31P & 1H NMR confirmation, and moisture analysis (Karl Fischer), to ensure consistency and reliability for automated synthesizers. Certificates of Analysis (COA) with full traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This product is hygroscopic (moisture-sensitive) and must be handled in a dry environment. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (Karl Fischer)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.