Description

3'-(2,2,5,5-Tetramethylpyrroline-1-Oxyl-3-Carbonyl)Amino-3'-Deoxyguanosine 5'-Triphosphate is a spin-labeled nucleotide analog, a critical tool for advanced biophysical and structural biology research. Its core value lies in enabling the study of nucleic acid dynamics, protein-nucleic acid interactions, and conformational changes through techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. This compound is essential for researchers and developers in academic institutions, pharmaceutical R&D, and biotechnology companies focused on mechanistic enzymology, drug discovery targeting nucleic acids, and the development of novel diagnostic assays.

Application

• EPR/ESR Spectroscopy: As a site-directed spin label (SDSL) for probing the structure, dynamics, and distances within DNA and RNA molecules.
• Nucleic Acid-Protein Interaction Studies: To investigate binding mechanisms, kinetics, and conformational changes upon complex formation.
• Enzymology Research: Used as a substrate or inhibitor analog to study the catalytic mechanisms of polymerases, helicases, nucleases, and other nucleic acid-modifying enzymes.
• Structural Biology: Aiding in the determination of macromolecular structures and dynamics through integration with other techniques like NMR and cryo-EM.
• Biophysical Assay Development: Enabling the creation of sensitive, non-radioactive assays for monitoring enzymatic activity or molecular interactions.
• Nucleic Acid Nanotechnology: Potentially used to introduce paramagnetic centers into designed DNA/RNA nanostructures for functionalization and sensing applications.

Basic Information

Item	Details
Product Name	3'-(2,2,5,5-Tetramethylpyrroline-1-Oxyl-3-Carbonyl)Amino-3'-Deoxyguanosine 5'-Triphosphate
CAS No.	105822-89-7
Molecular Formula	C₂₀H₂₈N₈O₁₇P₃
Molecular Weight	754.39 g/mol
Synonyms	3'-SL-dGTP; 3'-Spin-Labeled-2'-deoxyguanosine-5'-triphosphate; 3'-(TEMPO-carbonylamino)-3'-deoxy-dGTP; 3'-amino-3'-deoxy-dGTP TEMPO conjugate; Nitroxide-labeled dGTP; EPR-active dGTP; 3'-TEMPO-dGTP; 3'-(2,2,5,5-Tetramethyl-1-oxyl-3-pyrroline-3-carboxamido)-3'-deoxyguanosine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our 3'-(2,2,5,5-Tetramethylpyrroline-1-Oxyl-3-Carbonyl)Amino-3'-Deoxyguanosine 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, EPR spectroscopy to verify spin label integrity, and quantitative analysis of triphosphate content. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere and avoid repeated freeze-thaw cycles. Keep containers sealed when not in use.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (EPR)	Characteristic nitroxide radical signal present
Purity (HPLC)	≥ 95%
Assay (Nucleotide Content)	≥ 85% (by UV absorbance)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in aqueous buffers, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.