Description

5-Azido-2'-Deoxyuridine-5'-Monophosphate is a chemically modified nucleoside monophosphate analog, serving as a critical building block and biochemical probe in advanced life science research. Its value lies in its unique azido functional group, which enables bioorthogonal click chemistry for labeling, crosslinking, and studying nucleic acid metabolism and interactions. This compound is essential for researchers and developers in pharmaceutical R&D, chemical biology, and molecular diagnostics, particularly for investigating nucleoside metabolism, designing prodrugs, and developing novel therapeutic strategies.

Application

• Biochemical Research Probe: Used as a substrate analog to study the activity and mechanisms of enzymes like thymidine kinase and other nucleoside/nucleotide metabolizing enzymes.
• Click Chemistry Reagent: The azido group facilitates copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) for site-specific labeling and conjugation of nucleic acids.
• Antiviral & Anticancer Research: Investigated as a potential precursor or active metabolite in the development of nucleoside-based prodrugs targeting viral replication or cancer cell proliferation.
• DNA/RNA Synthesis & Modification: Incorporated into oligonucleotides via enzymatic or chemical synthesis to create functionalized nucleic acids for diagnostic assays, aptamer development, or structural studies.
• Photocrosslinking Studies: The azido group can be photolyzed to generate nitrenes for crosslinking experiments to map nucleic acid-protein interactions.
• Metabolic Pathway Analysis: Tracks the incorporation and fate of modified nucleosides in cellular systems to understand metabolic pathways and drug resistance mechanisms.

Basic Information

Product Name	5-Azido-2'-Deoxyuridine-5'-Monophosphate
CAS No.	105426-34-4
Molecular Formula	C10H12N5O8P
Molecular Weight	361.21 g/mol
Synonyms	5-Azido-dUMP; 5-Azido-2'-deoxyuridylic acid; 5-N3-dUMP; 5-Azidodeoxyuridine monophosphate; 5-Azido-2'-deoxyuridine 5'-(dihydrogen phosphate); 5-Azido-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione 5'-monophosphate; Azidothymidine monophosphate analog (structural); Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

We supply 5-Azido-2'-Deoxyuridine-5'-Monophosphate with a focus on high purity and batch-to-batch consistency for research applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request. Specifications are designed to meet the stringent requirements of pharmaceutical R&D and academic research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the primary container in a sealed secondary container with an appropriate desiccant. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥95% (Area %)
Water Content (KF)	≤5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.