Description

5'-O-(4,4'-Dimethoxytrityl)-N4-Benzoyl-5-Methyl-2'-Deoxycytidine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides. This compound provides a critical building block for the creation of modified DNA sequences, ensuring high coupling efficiency and protecting sensitive functional groups during automated synthesis. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of antisense oligonucleotides, primers, probes, and other advanced nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A fundamental phosphoramidite precursor for the automated synthesis of DNA strands containing 5-methylcytosine modifications.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs targeting genetic diseases.
• PCR Primer and Probe Manufacture: Enables the synthesis of high-purity, sequence-specific primers and fluorescent probes for diagnostic assays.
• siRNA and miRNA Synthesis: Serves as a protected monomer for constructing RNAi-based therapeutic agents.
• Structural Studies and Biochemistry: Employed in academic and industrial research to study nucleic acid structure, function, and protein interactions.
• Pharmaceutical Intermediate: A critical raw material in the cGMP-compliant production of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N4-Benzoyl-5-Methyl-2'-Deoxycytidine
CAS No.	104579-03-5
Molecular Formula	C39H37N3O7
Molecular Weight	659.73 g/mol
Synonyms	5-Methyl-dC(Bz)-DMTr; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-5-methyl-2'-deoxycytidine; 5-Me-dC-CE Phosphoramidite precursor; 5-Methyl-2'-deoxycytidine, N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; DMTr-5-Me-dC(Bz); Protected 5-Methyldeoxycytidine; dC(Me,Bz)-DMTr
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to stringent quality protocols to ensure batch-to-batch consistency and suitability for sensitive oligonucleotide synthesis. Each lot is subjected to comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation. We provide detailed Certificates of Analysis (COA) with every shipment, documenting critical parameters such as assay, moisture content, and identity. Our quality system supports compliance with relevant guidelines for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under a dry inert atmosphere or in a desiccator to maintain stability and prevent decomposition. For long-term storage, consider storing at -20°C under nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.