Description

5'-O-(4,4'-Dimethoxytrityl)-2'-O-Methyluridine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides. This compound matters because it provides the precise chemical functionality required for building modified RNA strands with enhanced stability and specificity. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic research institutions engaged in the development of antisense oligonucleotides, siRNA therapeutics, and diagnostic probes.

Application

• Solid-Phase Oligonucleotide Synthesis: A critical phosphoramidite precursor for the automated synthesis of 2'-O-methyl modified RNA sequences.
• Therapeutic Oligonucleotide Development: Used in the research and production of siRNA, antisense, and aptamer-based drugs where nuclease resistance is required.
• Diagnostic Probe Manufacturing: Serves as a building block for labeled probes used in FISH (Fluorescence In Situ Hybridization) and other molecular diagnostic assays.
• Structural Biology Studies: Enables the incorporation of site-specific 2'-O-methyl modifications to study RNA structure, function, and protein-RNA interactions.
• Process Development & Scale-Up: Supports cGMP manufacturing for clinical and commercial-stage oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-O-Methyluridine
CAS No.	103285-22-9
Molecular Formula	C33H34N2O8
Molecular Weight	586.63 g/mol
Synonyms	5'-O-DMT-2'-O-Methyluridine; 2'-O-Methyl-5'-O-(4,4'-dimethoxytrityl)uridine; DMT-2'-O-Me-Uridine; 1-[(2R,3R,4R,5R)-5-[[Bis(4-methoxyphenyl)-phenylmethoxy]methyl]-3,4-dihydroxy-4-methoxyoxolan-2-yl]pyrimidine-2,4-dione; 2'-O-Methyl-5'-O-(4,4'-dimethoxytriphenylmethyl)uridine; NSC 339539; Protected 2'-O-Methyluridine
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-O-Methyluridine is produced under strict quality management systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, NMR and MS identification, and moisture content determination. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under a dry inert atmosphere or in a controlled humidity environment to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.