Description

1,N6-Ethenoadenosine-5'-Diphosphate Sodium Salt is a chemically modified nucleotide analog, specifically an etheno-derivative of adenosine diphosphate. This compound is a valuable biochemical tool for research and diagnostic applications due to its unique fluorescent properties and role as a stable analog of ADP. It is primarily utilized by researchers in biochemistry, molecular biology, and enzymology. The product is supplied as a high-purity sodium salt to ensure stability and solubility in aqueous buffers.

Application

• Enzyme Kinetics & Inhibition Studies: Used as a substrate analog or competitive inhibitor for studying ATP/ADP-dependent enzymes, including kinases, dehydrogenases, and synthases.
• Fluorescent Labeling & Probes: Serves as a core component for developing fluorescent nucleotide probes due to the intrinsic fluorescence of the etheno bridge, useful in fluorescence polarization (FP) and FRET assays.
• Metabolic Pathway Research: Employed in investigations of purinergic signaling, energy metabolism, and nucleotide-interacting proteins.
• Diagnostic Assay Development: Acts as a critical reagent in the development of luminescent or colorimetric diagnostic kits for enzyme activity detection.
• Structural Biology: Used in X-ray crystallography and NMR studies to elucidate the binding sites and mechanisms of nucleotide-binding proteins.
• High-Throughput Screening (HTS): Applied in pharmaceutical HTS campaigns to identify novel modulators of ADP/ATP-binding targets.

Basic Information

Product Name	1,N6-Ethenoadenosine-5'-Diphosphate Sodium Salt
CAS No.	103213-52-1
Molecular Formula	C12H14N5O10P2Nax (x≈3)
Molecular Weight	Approx. 549.2 g/mol (Free acid)
Synonyms	ε-ADP; 1,N6-Etheno-ADP; Ethenoadenosine diphosphate; 1,N6-Ethenoadenosine 5'-diphosphate sodium salt; Adenosine, 1,N6-etheno-, 5'-(trihydrogen diphosphate), sodium salt; 1,N6-Etheno-5'-ADP; Fluorescent ADP analog
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research chemicals. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and absence of critical impurities. Certificates of Analysis (COA) detailing batch-specific results are available upon request. We adhere to a quality management system to ensure traceability and consistency for all our biochemical offerings.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.