Description

5-Bromo-2'-Deoxyuridine 5'-Triphosphate Sodium Salt is a modified nucleotide triphosphate analog, specifically a brominated derivative of deoxyuridine triphosphate. This compound is a critical reagent in molecular biology and biochemistry research, primarily utilized as a substrate for DNA polymerases. It is essential for researchers and professionals in the fields of molecular diagnostics, enzymology, and nucleic acid labeling. The product is supplied as a high-purity sodium salt to ensure stability and solubility in aqueous buffer systems.

Application

• DNA Polymerase Substrate: Used as a modified nucleotide triphosphate (dNTP) analog in enzymatic DNA synthesis and sequencing reactions.
• Mutagenesis Studies: Incorporated into DNA to induce targeted mutations and study DNA repair mechanisms.
• Biochemical Assays: Serves as a substrate for assaying the activity and fidelity of various DNA polymerases and reverse transcriptases.
• Nucleic Acid Labeling: Can be used in techniques for labeling DNA, particularly when a halogenated base is required for subsequent detection or crosslinking.
• Antiviral Research: Investigated for its potential to inhibit viral replication by being incorporated into viral DNA by viral polymerases.
• Radiochemical Precursor: Acts as a starting material for the synthesis of radiolabeled (e.g., ³²P) nucleotide triphosphates for sensitive detection methods.

Basic Information

Product Name	5-Bromo-2'-Deoxyuridine 5'-Triphosphate Sodium Salt
CAS No.	102212-99-7
Molecular Formula	C10H13BrN2O14P3 · xNa+
Molecular Weight	Contact for details
Synonyms	5-Bromo-2'-deoxyuridine-5'-triphosphate trisodium salt; BrdUTP; 5-Bromo-dUTP; 5-BrdUTP; 5-Bromo-2'-deoxyuridine 5'-(tetrahydrogen triphosphate); 5-Bromodeoxyuridine triphosphate; 5-Bromo-2'-deoxyuridine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our 5-Bromo-2'-Deoxyuridine 5'-Triphosphate Sodium Salt is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is analyzed to confirm identity, purity, and the absence of contaminating nucleases and phosphatases. A comprehensive Certificate of Analysis (COA) detailing purity (typically by HPLC), identity confirmation (UV, NMR), and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (UV)	Conforms
Purity (HPLC)	≥95%
Solubility	Soluble in water
pH (1% Solution)	6.0 - 8.0
Moisture Content	Contact for details
Nuclease Activity	Not detected
Phosphatase Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.