Description

3-(2-Isothiocyanatoethylcarbamoyl)-Proxyl is a specialized nitroxide spin label compound containing a reactive isothiocyanate functional group. This chemical matters for researchers and developers in the life sciences as it enables the site-specific covalent labeling of biomolecules for advanced Electron Paramagnetic Resonance (EPR) spectroscopy studies. Professionals in pharmaceutical R&D, structural biology, and biophysics need this high-purity reagent to investigate protein structure, dynamics, and conformational changes with precision.

Application

• Site-directed spin labeling (SDSL) for EPR/ESR spectroscopy to study protein folding and dynamics.
• Covalent modification of amino groups in peptides, proteins, and antibodies for structural analysis.
• Investigation of membrane protein structure and lipid interactions within model lipid bilayers.
• Development of molecular probes for studying intracellular redox environments and oxidative stress.
• Distance measurement in biomolecules using pulsed EPR techniques such as Double Electron-Electron Resonance (DEER).
• Research tool in biophysical chemistry for understanding enzyme mechanisms and macromolecular interactions.

Basic Information

Product Name	3-(2-Isothiocyanatoethylcarbamoyl)-Proxyl
CAS No.	100929-92-8
Molecular Formula	C12H18N3O3S
Molecular Weight	284.35 g/mol
Synonyms	3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxy, 2-Isothiocyanatoethylamide; 3-(2-Isothiocyanatoethylcarbamoyl)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; 3-(2-Isothiocyanatoethylcarbamoyl)-PROXYL; PROXYL-isothiocyanate; 3-Isothiocyanato-PROXYL; 3-ITC-PROXYL; Nitroxide spin label isothiocyanate; EPR spin label reagent
EINECS	Contact for details

Quality Control

Our 3-(2-Isothiocyanatoethylcarbamoyl)-Proxyl is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for sensitive spectroscopic applications. Each lot is analyzed to confirm identity, purity, and the integrity of the reactive isothiocyanate group. Certificates of Analysis (COA) detailing comprehensive test results are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed under an inert atmosphere (e.g., argon or nitrogen) after opening to prevent degradation. Keep away from strong acids and bases.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (EPR)	≥ 95% active nitroxide
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.