Description

3-[2-(2-Isothiocyanatoethoxy)Ethylcarbamoyl]-Proxyl is a specialized nitroxide spin label reagent featuring a reactive isothiocyanate group, enabling covalent conjugation to primary amines in biomolecules. This compound is critical for advanced Electron Paramagnetic Resonance (EPR) spectroscopy and site-directed spin labeling (SDSL) studies, providing precise structural and dynamic information on proteins and other macromolecules. It is essential for researchers and developers in structural biology, biophysics, and pharmaceutical R&D focused on protein conformation, dynamics, and interactions.

Application

• Site-Directed Spin Labeling (SDSL) for probing protein structure, folding, and conformational changes.
• Electron Paramagnetic Resonance (EPR) Spectroscopy as a stable nitroxide radical probe for distance measurements (DEER/PELDOR).
• Bioconjugation to antibodies, enzymes, or peptides for creating spin-labeled conjugates for diagnostic or research applications.
• Membrane Protein Studies to investigate dynamics and lipid interactions within lipid bilayers.
• Polymer & Material Science as a radical probe for studying polymer dynamics, cross-linking, and degradation mechanisms.
• Drug Discovery for mapping binding sites and studying ligand-receptor interactions via EPR.

Basic Information

Product Name	3-[2-(2-Isothiocyanatoethoxy)Ethylcarbamoyl]-Proxyl
CAS No.	100929-91-7
Molecular Formula	C₁₄H₂₂N₃O₄S
Molecular Weight	328.41 g/mol
Synonyms	3-(2-(2-Isothiocyanatoethoxy)ethylcarbamoyl)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; 3-Isothiocyanato-PROXYL; 3-(2-(2-Isothiocyanatoethoxy)ethylcarbamoyl)proxyl; 3-Isothiocyanato-2,2,5,5-tetramethyl-1-pyrrolidinyloxy derivative; 3-ITC-PROXYL; PROXYL isothiocyanate; Nitroxide spin label isothiocyanate; EPR spin label reagent
EINECS	Contact for details

Quality Control

Our 3-[2-(2-Isothiocyanatoethoxy)Ethylcarbamoyl]-Proxyl is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. All products undergo rigorous analytical testing, including HPLC for purity and EPR for radical integrity. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and assay is provided with each shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) under inert atmosphere for long-term stability. Due to its hygroscopic and light-sensitive nature, allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder or crystals
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (EPR)	≥ 90% radical content
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.