Description

5'-O-(4,4'-Dimethoxytrityl)-N4-Acetyl-2'-Deoxycytidine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides. This compound is specifically designed to prevent unwanted side reactions during automated solid-phase synthesis, ensuring high coupling efficiency and sequence fidelity. It is a critical building block for research and production in the pharmaceutical and biotechnology sectors, particularly for the development of antisense oligonucleotides, siRNA, and diagnostic probes.

Application

• Oligonucleotide Synthesis: Serves as a protected monomer (dC-Ac) for automated solid-phase DNA and RNA synthesis.
• Antisense Therapeutics: A vital raw material in the production of antisense oligonucleotides for targeted gene silencing.
• siRNA & miRNA Production: Used in the manufacture of short interfering RNA (siRNA) and microRNA (miRNA) for research and therapeutic applications.
• Diagnostic Probe Development: Essential for creating labeled DNA probes used in PCR, sequencing, and microarray technologies.
• Pharmaceutical Intermediates: Acts as a high-purity intermediate in the synthesis of nucleoside-based active pharmaceutical ingredients (APIs).
• Biochemical Research: Employed in academic and industrial R&D for studying nucleic acid chemistry and developing novel genetic tools.

Basic Information

Item	Details
Product Name	5'-O-(4,4'-Dimethoxytrityl)-N4-Acetyl-2'-Deoxycytidine
CAS No.	100898-63-3
Molecular Formula	C34H35N3O8
Molecular Weight	613.66 g/mol
Synonyms	N4-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine; dC(Ac)-DMTr; 5'-O-DMT-N4-acetyl-2'-deoxycytidine; DMTr-dC(Ac); 2'-Deoxycytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N4-acetyl-; N4-Ac-dC(DMTr); Protected Deoxycytidine; DNA Synthesis Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N4-Acetyl-2'-Deoxycytidine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research and cGMP-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under a dry, inert atmosphere to maintain stability. For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.