Description

5'-O-(4,4'-Dimethoxytrityl)-N4-Isobutyryl-2'-Deoxycytidine is a key protected nucleoside derivative, essential for the solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient construction of DNA sequences by protecting the 5'-hydroxyl and exocyclic amine functionalities during automated synthesis cycles. This high-purity building block is critically needed by pharmaceutical R&D teams, diagnostic developers, and biotechnology companies engaged in the production of antisense oligonucleotides, primers, probes, and other therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis: As a phosphoramidite precursor for the controlled incorporation of cytosine into custom DNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and aptamer-based therapies.
• Diagnostic Probe & Primer Synthesis: Fabrication of high-fidelity probes for PCR, qPCR, sequencing, and microarray applications.
• Research-Grade Oligo Production: Synthesis of primers and gene fragments for molecular biology and genetic engineering research.
• Scale-Up cGMP Manufacturing: Serves as a critical raw material in cGMP-compliant processes for clinical and commercial oligonucleotide drugs.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N4-Isobutyryl-2'-Deoxycytidine
CAS No.	100898-62-2
Molecular Formula	C36H39N3O7
Molecular Weight	625.72 g/mol
Synonyms	5'-O-DMT-N4-Ibu-dC; DMT-dC(Ibu) CE Phosphoramidite; N4-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine; 2'-Deoxycytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N4-(2-methyl-1-oxopropyl)-; dC(Ibu) DMT; Protected Deoxycytidine; DMT-dCib; DNA Synthesis Building Block
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N4-Isobutyryl-2'-Deoxycytidine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS). We provide full traceability and detailed Certificates of Analysis (COA) to support your regulatory and quality assurance requirements for research, diagnostic, and cGMP pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.