Description

Thymidylyl-(3'-5')-Thymidine Cyanoethyl Phosphotriester is a specialized nucleotide derivative, specifically a dinucleoside phosphotriester. This compound matters as a crucial synthetic intermediate and building block in the field of oligonucleotide synthesis, enabling the creation of specific DNA sequences. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications in antisense therapy development, PCR primer synthesis, and genetic research.

Application

• Oligonucleotide Synthesis: A key phosphotriester intermediate in the solid-phase and solution-phase synthesis of custom DNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides for therapeutic applications targeting specific genes.
• PCR Primer & Probe Manufacture: Serves as a building block for synthesizing high-purity primers and probes used in polymerase chain reaction (PCR) and molecular diagnostics.
• Genetic Research Tools: Essential for constructing labeled or modified DNA sequences used in hybridization studies and genomic analysis.
• Bioconjugation & Labeling: Acts as a starting material for introducing functional groups or labels (e.g., fluorophores, biotin) onto oligonucleotides.
• Academic & Industrial R&D: Employed in research laboratories for studying nucleic acid chemistry, enzyme mechanisms, and developing novel synthetic methodologies.

Basic Information

Product Name	Thymidylyl-(3'-5')-Thymidine Cyanoethyl Phosphotriester
CAS No.	18875-74-6
Molecular Formula	C27H35N4O15P
Molecular Weight	686.56 g/mol
Synonyms	dTpdT Cyanoethyl Phosphotriester; Thymidylyl(3'→5')thymidine 2-Cyanoethyl Phosphotriester; TpT CE Phosphotriester; 5'-O-Dimethoxytrityl-thymidylyl-(3'-5')-thymidine 3'-(2-cyanoethyl) phosphate; Dinucleoside Phosphotriester; DNA Dimer Building Block; 2-Cyanoethyl TpT Phosphotriester; Oligonucleotide Synthesis Intermediate
EINECS	Contact for details

Quality Control

Our Thymidylyl-(3'-5')-Thymidine Cyanoethyl Phosphotriester is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research and production applications. Quality is verified through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each batch to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This material is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.