Description

Bromodeoxyuridine Triphosphate (BrdUTP) is a halogenated thymidine triphosphate analog, a critical nucleotide derivative used in molecular biology and biochemistry. Its primary value lies in its ability to be incorporated into DNA in place of thymidine triphosphate (dTTP), serving as a powerful tool for labeling and detecting DNA synthesis. This compound is essential for researchers and manufacturers in life sciences, diagnostics, and pharmaceutical development, particularly for applications like cell proliferation assays, DNA sequencing, and apoptosis detection.

Application

• DNA Labeling & Detection: Used as a substrate for terminal deoxynucleotidyl transferase (TdT) in TUNEL assays for apoptosis detection and in nick translation for probe labeling.
• Cell Proliferation Studies: Incorporated into newly synthesized DNA during S-phase, allowing visualization and quantification of dividing cells via anti-BrdU antibodies.
• Next-Generation Sequencing (NGS): Can be utilized in certain sequencing-by-synthesis (SBS) platforms or as a modified nucleotide for specialized sequencing applications.
• Fluorescence In Situ Hybridization (FISH): Employed in the preparation of labeled nucleic acid probes for chromosomal and genetic analysis.
• PCR and cDNA Synthesis: Acts as a modified nucleotide to introduce specific tags or handles into amplified DNA products for downstream purification or detection.
• Antiviral & Anticancer Research: Investigated for its potential to inhibit viral replication or disrupt DNA synthesis in rapidly dividing cancer cells.
• Biochemical Research: Serves as a substrate for studying the kinetics and specificity of DNA polymerases and other nucleic acid-processing enzymes.

Basic Information

Product Name	Bromodeoxyuridine Triphosphate
CAS No.	18736-53-3
Molecular Formula	C10H15BrN3O13P3
Molecular Weight	590.06 g/mol
Synonyms	5-Bromo-2'-deoxyuridine-5'-triphosphate; BrdUTP; 5-Bromodeoxyuridine triphosphate; 5-Bromo-dUTP; 5-BrdUTP; BUdR triphosphate; Bromodeoxyuridylic acid triphosphate; Thymidine triphosphate, 5-bromo-2'-deoxy-
EINECS	Contact for details

Quality Control

Our Bromodeoxyuridine Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Anhydrous basis)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, TE buffer (pH 7.5-8.0)
Biological Activity	Suitable for enzyme incorporation (TdT, Polymerases)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.