Description

Deoxythymidine Triphosphate CAS NO 18423-43-3 is a high-purity nucleotide essential for DNA synthesis and amplification processes. Its primary value lies in providing a critical, reliable building block for molecular biology applications, ensuring accurate and efficient results. This product is indispensable for research institutions, diagnostic laboratories, and biotechnology companies engaged in PCR, DNA sequencing, and various enzymatic assays.

Application

• Polymerase Chain Reaction (PCR): As a key substrate (dTTP) for DNA polymerases in standard, quantitative, and reverse transcription PCR.
• DNA Sequencing: Used in Sanger (dideoxy) sequencing and next-generation sequencing platforms for template-directed DNA synthesis.
• cDNA Synthesis: A vital component in first-strand cDNA synthesis from mRNA templates using reverse transcriptase.
• Site-Directed Mutagenesis: Employed in enzymatic methods for introducing specific mutations into DNA sequences.
• DNA Labeling: Can be used in enzymatic incorporation of labeled nucleotides for probe generation.
• Enzymatic Assays: Serves as a substrate for assaying the activity of DNA polymerases, kinases, and other nucleic acid-modifying enzymes.
• Biochemical Research: Fundamental for studies on DNA replication, repair mechanisms, and enzyme kinetics.

Basic Information

Product Name	Deoxythymidine Triphosphate
CAS No.	18423-43-3
Molecular Formula	C10H17N2O14P3
Molecular Weight	482.17 g/mol
Synonyms	dTTP; 2'-Deoxythymidine 5'-triphosphate; Thymidine 5'-triphosphate; Deoxythymidine 5'-triphosphate; Thymidine triphosphate; dThTP; TTP; 5'-TTP; 2'-Deoxy-5'-TTP
EINECS	242-314-7

Quality Control

Our Deoxythymidine Triphosphate is manufactured under stringent quality systems to ensure it meets the exacting requirements of molecular biology. Each batch is tested for identity, purity, and absence of contaminants such as nucleases. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications including assay by HPLC, pH, and endotoxin levels where applicable. We support compliance with relevant quality frameworks for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms
Assay (HPLC)	≥ 98.0%
pH (in solution)	6.5 - 7.5
Water Content (KF)	≤ 5.0%
Endotoxin (LAL)	< 0.1 EU/µmol (if specified)
Solubility	Soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.