Description

5'-Cytidylic Acid, 2'-o-Methyl- is a chemically modified nucleotide monophosphate, specifically a methylated derivative of cytidine 5'-monophosphate. This modification is critical for enhancing the stability and nuclease resistance of oligonucleotides, making it a valuable building block in advanced therapeutic and diagnostic applications. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, and other nucleic acid-based technologies.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A key phosphoramidite monomer for constructing oligonucleotides with improved metabolic stability.
• Small Interfering RNA (siRNA) Production: Used to introduce stabilizing 2'-O-methyl modifications into the siRNA guide or passenger strand.
• Diagnostic Probe Development: Essential for creating high-affinity, nuclease-resistant probes for molecular diagnostics and FISH (Fluorescence In Situ Hybridization).
• Therapeutic Aptamer Research: Serves as a modified nucleotide in the selection and synthesis of aptamers for targeted drug delivery.
• Biochemical Research: Employed in studies of RNA structure, function, and protein-RNA interactions where enhanced stability is required.
• Pharmaceutical Intermediates: Acts as a critical intermediate in the GMP-compliant manufacturing of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-Cytidylic Acid, 2'-o-Methyl-
CAS No.	18422-43-0
Molecular Formula	C10H15N3O8P
Molecular Weight	336.22 g/mol
Synonyms	2'-O-Methylcytidine 5'-monophosphate; 2'-O-Methyl-CMP; 2'-O-Methylcytidylic acid; 5'-CMP-2'-O-methyl; Cytidine 5'-(dihydrogen phosphate), 2'-O-methyl-; 2'-OMe-CMP; 2'-Methylcytidine 5'-phosphate; 2'-O-Methylcytidine 5'-(dihydrogen phosphate)
EINECS	Contact for details

Quality Control

Our 5'-Cytidylic Acid, 2'-o-Methyl- is manufactured under stringent quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for pharmaceutical and life science research. Certificates of Analysis (COA) with detailed chromatographic data are provided and can be tailored to support regulatory filings (e.g., Drug Master File references).

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.