Description

3'-O,5'-o-Bis(Trifluoroacetyl)Thymidine is a key protected nucleoside derivative, specifically a thymidine molecule where the 3' and 5' hydroxyl groups are masked with trifluoroacetyl (TFA) protecting groups. This chemical modification is critical for enabling selective reactions during the multi-step synthesis of oligonucleotides, nucleoside analogs, and other complex biomolecules. It is an essential high-purity building block for pharmaceutical research and development, particularly in the fields of antiviral and anticancer drug discovery. The compound, identified by CAS No. 18354-10-4, is supplied to meet the stringent demands of advanced chemical synthesis.

Application

• Pharmaceutical Intermediate: A crucial protected precursor in the synthesis of novel nucleoside-based antiviral and anticancer agents.
• Oligonucleotide Synthesis: Used in solid-phase and solution-phase synthesis to build DNA/RNA sequences, where selective deprotection is required.
• Prodrug Development: Serves as a synthetic intermediate for creating prodrugs of nucleoside analogs to improve bioavailability and targeting.
• Biochemical Research: Employed in academic and industrial R&D for studying enzyme mechanisms, nucleic acid chemistry, and metabolic pathways.
• Labeled Compound Synthesis: Acts as a starting material for the preparation of isotopically labeled (e.g., 13C, 15N, 3H) nucleosides for tracer studies.
• Custom Chemical Synthesis: Provides a versatile scaffold for contract research organizations (CROs) and CDMOs to create custom-modified nucleosides.

Basic Information

Product Name	3'-O,5'-o-Bis(Trifluoroacetyl)Thymidine
CAS No.	18354-10-4
Molecular Formula	C14H13F6N2O7
Molecular Weight	426.26 g/mol
Synonyms	1-(2-Deoxy-3,5-bis-O-(trifluoroacetyl)-β-D-ribofuranosyl)-5-methylpyrimidine-2,4(1H,3H)-dione; Thymidine 3',5'-Bis(trifluoroacetate); 3',5'-Di-O-trifluoroacetylthymidine; 3',5'-Bis-O-(trifluoroacetyl)thymidine; NSC 122047; 2'-Deoxythymidine 3',5'-bis(trifluoroacetate); 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione 3',5'-bis(2,2,2-trifluoroacetate)
EINECS	Contact for details

Quality Control

Our 3'-O,5'-o-Bis(Trifluoroacetyl)Thymidine is manufactured under controlled conditions to ensure high chemical purity and batch-to-batch consistency, suitable for sensitive synthetic applications. All products undergo rigorous quality testing, including HPLC for purity and identity confirmation by NMR and MS. We provide comprehensive Certificates of Analysis (COA) with each batch, detailing all critical specifications. Our quality management system supports compliance with cGMP and ICH guidelines for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to prevent hydrolysis of the protecting groups. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH limits
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.