Description

22(R)-Hydroxycholesterol CAS NO 17954-98-2 is a high-purity, stereospecific oxysterol derivative that serves as a critical intermediate and reference standard in advanced biochemical research. This compound is of significant value for its role in studying cholesterol metabolism, liver X receptor (LXR) signaling pathways, and neurosteroid biosynthesis. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic manufacturers focused on metabolic disorders, cardiovascular disease, and neurodegenerative conditions.

Application

• Biochemical Research & Reference Standard: Used as a high-purity analytical standard for LC-MS, HPLC, and GC-MS to quantify oxysterols in biological samples.
• Pharmaceutical R&D: A key intermediate and tool compound for investigating LXR (Liver X Receptor) agonists/antagonists in drug discovery programs for atherosclerosis, diabetes, and inflammation.
• Metabolic Pathway Studies: Essential for elucidating the enzymatic pathways of cholesterol hydroxylation and bile acid synthesis in vitro and in vivo.
• Cell Signaling & Receptor Studies: Employed to activate and study specific nuclear receptors (LXRs) to understand gene regulation related to lipid homeostasis.
• Neuroscience Research: Investigated for its potential role as a neurosteroid precursor and its effects on neuronal differentiation and survival.
• Diagnostic Kit Development: Serves as a critical calibrant and component in the development of ELISA and other immunoassays for oxysterol detection.

Basic Information

Product Name	22(R)-Hydroxycholesterol
CAS No.	17954-98-2
Molecular Formula	C27H46O2
Molecular Weight	402.66 g/mol
Synonyms	(22R)-Cholest-5-ene-3β,22-diol; 22R-Hydroxycholesterol; (22R)-22-Hydroxycholesterol; 3β,22R-Dihydroxycholest-5-ene; 22(R)-OHC; 22R-OH-Cholesterol; 22R-HC; 5-Cholestene-3β,22(R)-diol
EINECS	Contact for details

Quality Control

Our 22(R)-Hydroxycholesterol is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis to confirm stereochemical integrity, and identity confirmation by IR and MS. We provide full traceability and Certificates of Analysis (COA) detailing all specifications. The product is suitable for use as a reference material in pharmaceutical and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. For long-term storage, keep desiccated to maintain stability. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Chiral Purity (Chiral HPLC)	≥ 99.0% (22R isomer)
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.