Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine is a critical nucleoside phosphoramidite precursor, essential for the automated solid-phase synthesis of oligonucleotides. This compound matters because it provides the protected adenine base necessary for constructing DNA sequences with high coupling efficiency and purity. It is primarily needed by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for applications in antisense therapy, PCR primers, gene synthesis, and probe development.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite building block in automated DNA synthesizers.
• Therapeutic Agent Development: For the production of antisense oligonucleotides, siRNA, and aptamers for targeted therapies.
• Molecular Diagnostics: Manufacture of primers, probes, and other components for PCR, qPCR, and sequencing assays.
• Genomic Research: Synthesis of gene fragments, libraries, and tools for genetic engineering and functional studies.
• Bioconjugation: Intermediate for attaching functional groups or labels to oligonucleotides.
• Scale-Up cGMP Manufacturing: Serves as a starting material for the production of clinical-grade oligonucleotide APIs under Good Manufacturing Practice (GMP) guidelines.

Basic Information

Item	Details
Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine
CAS No.	17331-22-5
Molecular Formula	C31H31N5O5
Molecular Weight	553.61 g/mol
Synonyms	5'-O-DMT-2'-deoxyadenosine; DMT-dA; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine (common protected form); 2'-Deoxyadenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; DMTr-dA; 5'-O-(Bis(4-methoxyphenyl)phenylmethyl)-2'-deoxyadenosine; dA(DMT)
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine is produced under stringent quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity verification. We provide full traceability and detailed Certificates of Analysis (COA) with every shipment. Our quality commitment supports applications requiring high purity, from research to cGMP manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This material is hygroscopic (moisture-sensitive); ensure containers are sealed under an inert atmosphere (e.g., argon or nitrogen) after opening to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.