Description

12α-Hydroxy-3,7-Dioxo-5α-Cholan-24-Oic Acid Methyl Ester is a high-purity, synthetic bile acid derivative that serves as a critical advanced intermediate in pharmaceutical research and development. This compound is valued for its defined stereochemistry and functional groups, which make it an essential building block for synthesizing novel therapeutic agents and reference standards. It is primarily sought by research institutions and pharmaceutical companies engaged in the development of treatments for metabolic, hepatic, and gastrointestinal disorders.

Application

• Pharmaceutical Intermediate: A key precursor in the multi-step synthesis of complex steroid-based active pharmaceutical ingredients (APIs).
• Reference Standard: Used as a certified reference material (CRM) for analytical method development, validation, and quality control in pharmaceutical laboratories.
• Metabolic Research: Employed in biochemical studies to investigate bile acid metabolism, liver function, and related metabolic pathways.
• Medicinal Chemistry: Serves as a scaffold for structure-activity relationship (SAR) studies aimed at discovering new drug candidates targeting nuclear receptors like FXR.
• Process Development: Utilized in scaling up and optimizing synthetic routes for the production of cholanic acid derivatives under Good Manufacturing Practice (GMP) conditions.

Basic Information

Product Name	12α-Hydroxy-3,7-Dioxo-5α-Cholan-24-Oic Acid Methyl Ester
CAS No.	16656-66-9
Molecular Formula	C25H38O5
Molecular Weight	418.57 g/mol
Synonyms	Methyl 12α-Hydroxy-3,7-dioxo-5α-cholan-24-oate; 12α-Hydroxy-3,7-dioxolithocholic Acid Methyl Ester; Methyl 3,7,12-Trioxo-5α-cholan-24-oate (tautomeric form); 5α-Cholan-24-oic Acid, 12α-Hydroxy-3,7-dioxo-, Methyl Ester; 12α-Hydroxylithocholic Acid Methyl Ester Diketone Derivative; 12α-Hydroxy-3,7-diketolithocholate Methyl Ester
EINECS	Contact for details

Quality Control

Our 12α-Hydroxy-3,7-Dioxo-5α-Cholan-24-Oic Acid Methyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the stringent requirements for pharmaceutical research. A detailed Certificate of Analysis (COA) is provided with every shipment to guarantee traceability, consistency, and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The compound should be kept under an inert atmosphere if long-term storage is required to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Specific Optical Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.