Description

4-Nitrophenyl 3'-Thymidylate is a specialized nucleotide derivative, a key building block in oligonucleotide synthesis and biochemical research. Its primary value lies in its role as a protected monomer for the controlled assembly of DNA sequences, enabling precise genetic and diagnostic applications. This high-purity intermediate is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and molecular diagnostics sectors who require reliable, high-quality reagents for advanced nucleic acid chemistry.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of custom DNA strands and probes.
• Biochemical Research: Used in enzymatic studies, as a substrate analog, and in the investigation of nucleotide metabolism pathways.
• Diagnostic Reagent Manufacturing: Serves as a raw material for producing labeled nucleotides used in PCR, sequencing, and microarray technologies.
• Antisense & Therapeutic Development: A foundational component in the research and production of antisense oligonucleotides and other nucleic acid-based therapeutics.
• Academic & Industrial R&D: Employed in laboratories for method development, catalysis studies, and exploring novel bioconjugation techniques.

Basic Information

Product Name	4-Nitrophenyl 3'-Thymidylate
CAS No.	16562-50-8
Molecular Formula	C16H17N3O9P
Molecular Weight	435.30 g/mol
Synonyms	Thymidine 3'-(4-nitrophenyl phosphate); 3'-Thymidylic acid 4-nitrophenyl ester; p-Nitrophenyl thymidine 3'-phosphate; 4-Nitrophenyl thymidine-3'-monophosphate; 4-Nitrophenyl 3'-TMP; 4-Nitrophenyl 3'-thymidine monophosphate; 3'-(4-Nitrophenyl phosphate)thymidine; 16562-50-8
EINECS	Contact for details

Quality Control

Our production of 4-Nitrophenyl 3'-Thymidylate adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity suitable for sensitive research and synthesis applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.