Description

n-Hydroxysuccinimidyl Chloroformate is a highly reactive, bifunctional cross-linking agent primarily used for the synthesis of active esters. This compound matters for its efficiency in facilitating peptide coupling and bioconjugation reactions under mild conditions, enabling the stable attachment of molecules to surfaces, polymers, or biomolecules. It is critically needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostics industries for developing advanced drug delivery systems, immunoassays, and functionalized materials.

Application

• Peptide Synthesis: As an efficient coupling reagent for forming amide bonds in solid-phase and solution-phase peptide synthesis.
• Bioconjugation & Labeling: For covalently attaching proteins, antibodies, or other biomolecules to fluorescent dyes, enzymes, or solid supports (e.g., beads, plates, chips).
• Polymer Functionalization: Introducing reactive N-hydroxysuccinimide (NHS) ester groups onto polymer backbones for subsequent grafting of bioactive molecules.
• Surface Modification: Creating functionalized surfaces on gold, silicon, or polymers for biosensor development and microarray fabrication.
• Cross-linking Agent: For creating cross-linked hydrogels or protein-protein conjugates used in tissue engineering and therapeutic applications.
• Intermediate for Reagent Kits: Serving as a key raw material in the production of commercial cross-linking and labeling kits for life science research.

Basic Information

Product Name	n-Hydroxysuccinimidyl Chloroformate
CAS No.	15149-73-2
Molecular Formula	C6H4ClNO5
Molecular Weight	205.55 g/mol
Synonyms	N-Hydroxysuccinimidyl Chloroformate; Succinimidyl Chloroformate; NSC 1084; NSC 108401; Chloroformic Acid N-Hydroxysuccinimide Ester; 1-[(Chlorocarbonyl)oxy]-2,5-pyrrolidinedione; HOSu Chloroformate; N-Hydroxy-2,5-pyrrolidinedione Chloroformate
EINECS	Contact for details

Quality Control

Our n-Hydroxysuccinimidyl Chloroformate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and production applications. Each lot is analyzed using advanced techniques including HPLC, NMR, and FTIR. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to prevent decomposition. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.