Description

3β-(β-D-Glucopyranosyloxy)-14-Hydroxybufa-4,20,22-Trienolide CAS NO 14904-71-3 is a specialized cardiac glycoside derivative, a high-purity reference standard and intermediate of significant interest in pharmaceutical research and development. Its core value lies in its precise chemical structure, which is critical for analytical method development, quality control of related drug substances, and advanced pharmacological studies. This compound is essential for research laboratories, pharmaceutical manufacturers, and academic institutions focused on cardiovascular drug discovery, toxicology, and the synthesis of novel therapeutic agents.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the identification, assay, and impurity profiling of bufadienolide-based APIs and finished drug products.
• Active Pharmaceutical Ingredient (API) Intermediate: Serves as a key synthetic precursor in the manufacture of specialized cardiac glycosides and related therapeutic compounds.
• Biochemical Research: Employed in in vitro and in vivo studies to investigate mechanisms of action, structure-activity relationships (SAR), and metabolic pathways of cardiac steroids.
• Analytical Method Development: Critical for developing and validating HPLC, LC-MS, and other chromatographic methods for quality control laboratories.
• Toxicology and Safety Assessment: Used as a standard in assays to study the toxicity, pharmacokinetics, and pharmacodynamics of bufadienolide compounds.

Basic Information

Product Name	3β-(β-D-Glucopyranosyloxy)-14-Hydroxybufa-4,20,22-Trienolide
CAS No.	14904-71-3
Molecular Formula	C30H42O9
Molecular Weight	546.65 g/mol
Synonyms	14-Hydroxybufalin 3-O-β-D-glucopyranoside; Bufalin 3-O-β-D-glucoside; 3β-(β-D-Glucopyranosyloxy)-14-hydroxybufa-4,20,22-trienolide; Gamabufotalin 3-O-β-D-glucoside; 14-Hydroxy-3β-(β-D-glucopyranosyloxy)bufa-4,20,22-trienolide; Bufadienolide glycoside; Cardiac glycoside derivative
EINECS	Contact for details

Quality Control

Our 3β-(β-D-Glucopyranosyloxy)-14-Hydroxybufa-4,20,22-Trienolide is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity, IR and MS for structural confirmation, and residual solvent analysis. We provide full traceability and Certificates of Analysis (COA) detailing all test results to ensure compliance with research and pharmaceutical-grade standards. Custom purity grades and specific impurity profiles are available to meet your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and handled under appropriate conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.