Description

5'-O-Methylthymidine is a chemically modified nucleoside derivative, specifically a methylated analog of thymidine. This compound is a crucial building block and intermediate in organic synthesis and pharmaceutical research, valued for its role in modulating the properties of oligonucleotides. It is primarily utilized by researchers and manufacturers in the fields of nucleoside chemistry, medicinal chemistry, and oligonucleotide synthesis for developing novel therapeutics and diagnostic probes.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of modified DNA/RNA strands for research and therapeutic applications.
• Antiviral & Anticancer Drug Development: Serves as a critical scaffold and intermediate in the synthesis of nucleoside analog prodrugs.
• Medicinal Chemistry Research: Used to study structure-activity relationships (SAR) by introducing methylation at the sugar moiety to alter pharmacokinetic properties.
• Biochemical Probes: Incorporated into oligonucleotides to create probes resistant to nuclease degradation for diagnostic assays.
• Academic & Industrial R&D: A fundamental reagent for exploring novel nucleoside chemistry and enzymology in university and corporate laboratories.

Basic Information

Product Name	5'-O-Methylthymidine
CAS No.	14504-60-0
Molecular Formula	C11H16N2O5
Molecular Weight	256.26 g/mol
Synonyms	1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)-4-methoxytetrahydrofuran-2-yl]-5-methylpyrimidine-2,4(1H,3H)-dione; 5'-O-Methylthymidine; Thymidine, 5'-O-methyl-; 1-(5-O-Methyl-β-D-ribofuranosyl)thymine; 5'-Methylthymidine
EINECS	Contact for details

Quality Control

Our 5'-O-Methylthymidine is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and residual solvent analysis, to ensure it meets the high standards required for pharmaceutical research and synthesis. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions when possible.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.